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http://hdl.handle.net/2445/130889
Title: | Ability of a urine gene expression classifier to reduce the number of follow up cystoscopies in bladder cancer patients |
Author: | Montalbo Calafell, Ruth Lozano Salvatella, Juan José Izquierdo Reyes, Laura Ingelmo-Torres, Mercedes Baños, Carmen Palou, Joan Heijden, Antoine G. van der Medina, Rafael Schmidbauer, Joerg Prat Aparicio, Aleix Ribal, María José Alcaraz Asensio, Antonio Mengual Brichs, Lourdes |
Keywords: | Diagnòstic per la imatge Càncer de bufeta Expressió gènica Diagnostic imaging Bladder cancer Gene expression |
Issue Date: | 7-Feb-2019 |
Publisher: | Elsevier |
Abstract: | This study aimed to improve our previous urine gene expression classifiers focusing on the detection of non-high-risk non-muscle-invasive bladder cancer (NMIBC), and develop a new classifier able to decrease the frequency of cystoscopies during bladder cancer (BC) patients' surveillance. A total of 597 urines from BC patients, controls and patients in follow-up for BC (PFBC) were included. The study has 3 phases. In the urinary biomarker discovery phase, 84 urines from BC and control patients were retrospectively included and analyzed by Ribonucleic Acid (RNA) sequencing. In the classifier development phase, a total of 132 selected genes from previous phase were evaluated by nCounter in 214 prospectively collected urines from PFBC (98 with tumor). A diagnostic classifier was generated by logistic regression. Finally, in the classifier validation phase, a multicentric and international cohort of 248 urines (134 BC and 114 nonrecurrent PFBC) was used to validate classifier performance. A total of 521 genes were found differentially expressed between non-high-risk NMIBC samples and all other groups (P < 0.05). An 8-gene diagnostic classifier with an area under curve (AUC) of 0.893 was developed. Validation of this classifier in a cohort of PFBC achieved an overall sensitivity (SN) and a negative predictive value (NPV) of 96% and 97%, respectively (AUC = 0.823). Notably, this accuracy was maintained in non-high-risk NMIBC group (SN = 94%; NPV = 98%). In conclusion, this 8-gene expression classifier has high SN and NPV in a real clinical scenario. The use of this classifier can reduce the number of follow-up cystoscopies in PFBC, although assessing its final place in clinical setting is necessary. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1016/j.trsl.2019.02.003 |
It is part of: | Translational Research, The Journal of Laboratory and Clinical Medicine, 2019, vol. S1931-5244, num. 19, p. 30025-30028 |
URI: | http://hdl.handle.net/2445/130889 |
Related resource: | https://doi.org/10.1016/j.trsl.2019.02.003 |
ISSN: | 1931-5244 |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques) |
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