Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/134283
Full metadata record
DC FieldValueLanguage
dc.contributor.authorTorrejón-Escribano, Benjamín-
dc.contributor.authorEscoriza, Jessica-
dc.contributor.authorMontanya Mias, Eduard-
dc.contributor.authorBlasi Cabús, Joan-
dc.date.accessioned2019-05-31T13:06:25Z-
dc.date.available2019-05-31T13:06:25Z-
dc.date.issued2011-04-
dc.identifier.issn0013-7227-
dc.identifier.urihttp://hdl.handle.net/2445/134283-
dc.description.abstractProlonged exposure to high glucose concentration alters the expression of a set of proteins in pancreatic-cells and impairs their capacity to secrete insulin. The cellular and molecular mechanisms that lie behind this effect are poorly understood. In this study, three either in vitro or in vivo models (cultured rat pancreatic islets incubated in high glucose media, partially pancreatectomized rats, and islets transplanted to streptozotozin-induced diabetic mice) were used to evaluate the dependence of the biological model and the treatment, together with the cell location (insulin granule or plasma membrane) of the affected proteins and the possible effect of sustained insulin secretion, on the glucose-induced changes in protein expression. In all three models, islets exposed to high glucose concentrations showed a reduced expression of secretory granule associated vesicle-soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins synaptobrevin/ vesicle-associated membrane protein 2 and cellubrevin but minor or no significant changes in the expression of the membrane-associated target-SNARE proteins syntaxin1 and synaptosomal associated protein-25 and a marked increase in the expression of synaptosomal-associated protein-23 protein. The inhibition of insulin secretion by the L-type voltage-dependent calcium channel nifedipine or the potassium channel activator diazoxide prevented the glucoseinduced reduction in islet insulin content but not in vesicle-SNARE proteins, indicating that the granule depletion due to sustained exocytosis was not involved in the changes of protein expression induced by high glucose concentration. Altogether, the results suggest that high glucose has a direct toxic effect on the secretory pathway by decreasing the expression of insulin granule SNARE-associated proteins.-
dc.format.extent10 p.-
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherAssociation for the Study of Internal Secretions-
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1210/en.2010-0898-
dc.relation.ispartofEndocrinology, 2011, vol. 152, num. 4, p. 1290-1299-
dc.relation.urihttps://doi.org/10.1210/en.2010-0898-
dc.rights(c) Association for the Study of Internal Secretions, 2011-
dc.subject.classificationGlucosa-
dc.subject.classificationFarmacologia-
dc.subject.classificationInsulina-
dc.subject.classificationFarmacocinètica-
dc.subject.classificationMetabolisme-
dc.subject.classificationProteïnes SNARE-
dc.subject.classificationCèl·lules B-
dc.subject.otherGlucose-
dc.subject.otherPharmacology-
dc.subject.otherInsulin-
dc.subject.otherPharmacokinetics-
dc.subject.otherMetabolism-
dc.subject.otherSNARE Proteins-
dc.subject.otherB cells-
dc.titleGlucose-dependent changes in SNARE proyein levels in pancreatic beta-cells-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.identifier.idgrec584596-
dc.date.updated2019-05-31T13:06:25Z-
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess-
dc.identifier.pmid21285315-
Appears in Collections:Articles publicats en revistes (Ciències Clíniques)
Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

Files in This Item:
File Description SizeFormat 
584596.pdf2.4 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.