Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/134641
Title: Increased survival of dopaminergic neurons in striatal grafts of fetal ventral mesencephalic cells exposed to neurotrophin-3 or glial cell line- derived neurotrophic factor
Author: Espejo, Mónica
Cutillas Arroyo, Blanca
Arenas, Ernest
Ambrosio Viale, Santiago
Keywords: Trasplantament d'òrgans
Cirurgia
Factors de creixement
Teixit nerviós
Neurones
Transplantation of organs
Surgery
Growth factors
Nerve tissue
Neurons
Issue Date: Feb-2000
Publisher: Cognizant Communication Corporation
Abstract: The transplantation of fetal mesencephalic cell suspensions into the brain striatal system is an emerging treatment for Parkinson's disease. However, one objection to this procedure is the relatively poor survival of implanted cells. The ability of neurotrophic factors to regulate developmental neuron survival and differentiation suggests they could be used to enhance the success of cerebral grafts. We studied the effects of neurotrophin-3 (NT-3) or glial cell line-derived neurotrophic factor (GDNF) on the survival of dopaminergic neurons from rat fetal ventral mesencephalic cells (FMCs) implanted into the rat striatum. Two conditions were tested: (a) incubation of FMCs in media containing NT-3 and GDNF, prior to grafting, and (b) co-grafting of FMCs with cells engineered to overexpress high levels of NT-3 or GDNF. One week after grafting into the rat striatum, the survival of TH+ neurons was significantly increased by pretreatment of ventral mesencephalic cells with NT-3 or GDNF. Similarly, co-graft of ventral mesencephalic cells with NT-3- or GDNF-overexpressing cells, but not the mock-transfected control cell line, increased the survival of graft-derived dopaminergic neurons. Interestingly, we also found that co-grafting of GDNF-overexpressing cells was less effective than NT-3 at improving the survival of fetal dopaminergic neurons in the grafts, and that only GDNF induced intense TH immunostaining in fibers and nerve endings of the host tissue surrounding the implant. Thus, our results suggest that NT-3, by strongly enhancing survival, and GDNF, by promoting both survival and sprouting, may improve the efficiency of fetal transplants in the treatment of Parkinson's disease.
Note: Reproducció del document publicat a: https://doi.org/10.1177/096368970000900107
It is part of: Cell Transplantation, 2000, vol. 9, num. 1, p. 45-53
URI: http://hdl.handle.net/2445/134641
Related resource: https://doi.org/10.1177/096368970000900107
ISSN: 0963-6897
Appears in Collections:Articles publicats en revistes (Infermeria Fonamental i Medicoquirúrgica)
Articles publicats en revistes (Ciències Fisiològiques)

Files in This Item:
File Description SizeFormat 
169959.pdf2.82 MBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons