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http://hdl.handle.net/2445/134789
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DC Field | Value | Language |
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dc.contributor.author | Estil·les Altimiras, Elisabet | - |
dc.contributor.author | Téllez i Besolí, Noèlia | - |
dc.contributor.author | Escoriza, Jessica | - |
dc.contributor.author | Montanya Mias, Eduard | - |
dc.date.accessioned | 2019-06-07T17:03:43Z | - |
dc.date.available | 2019-06-07T17:03:43Z | - |
dc.date.issued | 2012-10 | - |
dc.identifier.issn | 0963-6897 | - |
dc.identifier.uri | http://hdl.handle.net/2445/134789 | - |
dc.description.abstract | Insulin-like growth factor II (IGF2) is a growth-promoting peptide that increases β-cell proliferation and survival. The aim of the study was to determine the effect of IGF2 overexpression on β-cell mass in transplanted islets. Islets infected with adenovirus encoding for IGF2 (Ad-IGF2 group), for luciferase (Ad-Luc control group), or with uninfected islets (control group) were syngeneically transplanted to streptozotocin-diabetic Lewis rats. Eight hundred islets, a minimal mass model to restore normoglycemia, or 500 islets, a clearly insufficient mass, were transplanted. Rats transplanted with 800 Ad-IGF2 islets showed a better metabolic evolution than control groups. As expected, rats transplanted with 500 Ad-IGF2 or control islets maintained similar hyperglycemia throughout the study, ensuring comparable metabolic conditions among both groups. β-Cell replication was higher in Ad-IGF2 group than in control group on days 3 [1.45% (IQR: 0.26) vs. 0.58% (IQR: 0.18), p = 0.006], 10 [1.58% (IQR: 1.40) vs. 0.90% (IQR: 0.61), p = 0.035], and 28 [1.35% (IQR: 0.35) vs. 0.64% (IQR: 0.28), p = 0.004] after transplantation. β-Cell mass was similarly reduced on day 3 after transplantation in Ad-IGF2 and control group [0.36 mg (IQR: 0.26) vs. 0.38 mg (IQR: 0.19)], it increased on day 10, and on day 28 it was higher in Ad-IGF2 than in control group [0.63 mg (IQR: 0.38) vs. 0.42 mg (IQR: 0.31), p = 0.008]. Apoptosis was similarly increased in Ad-IGF2 and control islets after transplantation. No differences in insulin secretion were found between Ad-IGF2 and uninfected control islets. In summary, IGF2 overexpression in transplanted islets increased β-cell replication, induced the regeneration of the transplanted β-cell mass, and had a beneficial effect on the metabolic outcome reducing the β-cell mass needed to achieve normoglycemia. | - |
dc.format.extent | 11 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Cognizant Communication Corporation | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3727/096368912X638955 | - |
dc.relation.ispartof | Cell Transplantation, 2012, vol. 21, num. 10, p. 2119-2129 | - |
dc.relation.uri | https://doi.org/10.3727/096368912X638955 | - |
dc.rights | cc-by-nc (c) Cognizant Communication Corporation, 2012 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0/es | - |
dc.source | Articles publicats en revistes (Ciències Clíniques) | - |
dc.subject.classification | Diabetis | - |
dc.subject.classification | Insulina | - |
dc.subject.classification | Biosíntesi | - |
dc.subject.classification | Illots de Langerhans | - |
dc.subject.classification | Regeneració (Biologia) | - |
dc.subject.classification | Cèl·lules B | - |
dc.subject.other | Diabetes | - |
dc.subject.other | Insulin | - |
dc.subject.other | Biosynthesis | - |
dc.subject.other | Islands of Langerhans | - |
dc.subject.other | Regeneration (Biology) | - |
dc.subject.other | B cells | - |
dc.title | Increased beta cell replication, and beta cell mass regeneration in syngeneically transplanted rat islets overexpressing insulin-like growth factor-II | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 622059 | - |
dc.date.updated | 2019-06-07T17:03:43Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 22507193 | - |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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622059.pdf | 396.94 kB | Adobe PDF | View/Open |
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