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Title: Characterization of glycoproteins by capillary electrophoresis electrospray mass spectrometry (CE-ESI-MS). Applications to diagnosis in biomedicine
Author: Barbosa Torralbo, José
Sanz Nebot, María Victoria
Benavente Moreno, Fernando J. (Julián)
Giménez López, Estela
Keywords: Electroforesi capil·lar
Espectrometria de masses
Capillary electrophoresis
Mass spectrometry
Issue Date: 1-Jun-2006
Abstract: Glycosylation is the most common posttranslational modification in proteins and the carbohydrates participate in many biological processes. The number and type of glycoforms for a certain glycoprotein may change as a consequence of pathological processes. In our work a method for the separation of transferrin sialoforms has been developed, that permits the diagnostic of Congenital Disorders of Glycosylation (CDG) using a polybrene-dextran sulphate coating and CE-ESI-TOF methodologies. In order to improve the sensitivity the use of solid-phase extraction coupled on-line to CE-ESI-MS is studied and the SPE-CE-ESI-MS developed methods are applied for the characterization of rHuEPO glycoforms. The achieved separation and the high mass-resolving power of flight (TOF) mass detection allows to establish the most probable rHuEPO glycoforms.
It is part of: FABAD Journal of Pharmaceutical Sciences, 2006, vol. 31, p. 63-69
Appears in Collections:Articles publicats en revistes (Enginyeria Química i Química Analítica)

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