Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/135009
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Biarnés Costa, Montse | - |
dc.contributor.author | Montolio Rusiñol, Marta | - |
dc.contributor.author | Nacher, Victor | - |
dc.contributor.author | Raurell, Mercè | - |
dc.contributor.author | Soler Ramon, Joan | - |
dc.contributor.author | Montanya Mias, Eduard | - |
dc.date.accessioned | 2019-06-13T15:05:05Z | - |
dc.date.available | 2019-06-13T15:05:05Z | - |
dc.date.issued | 2002-01 | - |
dc.identifier.issn | 0012-1797 | - |
dc.identifier.uri | http://hdl.handle.net/2445/135009 | - |
dc.description.abstract | We studied the effects of hyperglycemia on beta-cell death and mass in syngeneically transplanted islets. Six groups of STZ-induced diabetic C57BL/6 mice were transplanted with 100 syngeneic islets, an insufficient beta-cell mass to restore normoglycemia. Groups 1, 2, and 3 remained hyperglycemic throughout the study. Groups 4, 5, and 6 were treated with insulin from day 7 before transplantation to day 10 after transplantation. After insulin discontinuation, group 6 mice achieved definitive normoglycemia. Grafts were harvested at 3 (groups 1 and 4), 10 (groups 2 and 5), and 30 (groups 3 and 6) days after transplantation. On day 3, the initially transplanted beta-cell mass (0.13 +/- 0.01 mg) was dramatically and similarly reduced in the hyperglycemic and insulin-treated groups (group 1: 0.048 +/- 0.002 mg; group 4: 0.046 +/- 0.007 mg; P < 0.001). Extensive islet necrosis (group 1: 30.7%; group 4: 26.8%) and increased beta-cell apoptosis (group 1: 0.30 +/- 0.05%; group 4: 0.42 +/- 0.07%) were found. On day 10, apoptosis remained increased in both hyperglycemic and insulin-treated mice (group 2: 0.44 +/- 0.09%; group 5: 0.48 +/- 0.08%) compared with normal pancreas (0.04 +/- 0.03%; P < 0.001). In contrast, on day 30, beta-cell apoptosis was increased in grafts exposed to sustained hyperglycemia (group 3: 0.37 +/- 0.03%) but not in normoglycemic mice (group 6: 0.12 +/- 0.02%); beta-cell mass was selectively reduced in islets exposed to hyperglycemia (group 3: 0.046 +/- 0.02 mg; group 6: 0.102 +/- 0.009 mg; P < 0.01). In summary, even in optimal conditions, approximately 60% of transplanted islet tissue was lost 3 days after syngeneic transplantation, and both apoptosis and necrosis contributed to beta-cell death. Increased apoptosis and reduced beta-cell mass were also found in islets exposed to chronic hyperglycemia, suggesting that sustained hyperglycemia increased apoptosis in transplanted beta-cells. | - |
dc.format.extent | 7 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Diabetes Association | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.2337/diabetes.51.1.66 | - |
dc.relation.ispartof | Diabetes, 2002, vol. 51, num. 1, p. 66-72 | - |
dc.relation.uri | https://doi.org/10.2337/diabetes.51.1.66 | - |
dc.rights | cc-by-nc-nd (c) American Diabetes Association, 2002 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es | - |
dc.source | Articles publicats en revistes (Ciències Clíniques) | - |
dc.subject.classification | Diabetis | - |
dc.subject.classification | Cirurgia experimental | - |
dc.subject.classification | Hiperglucèmia | - |
dc.subject.classification | Illots de Langerhans | - |
dc.subject.other | Diabetes | - |
dc.subject.other | Experimental surgery | - |
dc.subject.other | Hyperglycemia | - |
dc.subject.other | Islands of Langerhans | - |
dc.title | B-cell death and mass in syngeneically transplanted islets exposed to short and long-term hyperglycemia | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 537266 | - |
dc.date.updated | 2019-06-13T15:05:05Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 11756324 | - |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
537266.pdf | 166.53 kB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License