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Title: Evaluation of the MagicplexTM sepsis real-time test for the rapid diagnosis of bloodstream infections in adults
Author: Zboromyrska,Yuliya
Cillóniz, Catia
Cobos-Trigueros, Nazaret
Almela, M. (Manel)
Hurtado, Juan Carlos
Vergara Gómez, Andrea
Mata, Caterina
Soriano Viladomiu, Alex
Mensa Pueyo, Josep
Marco Reverté, Francesc
Vila Estapé, Jordi
Keywords: Septicèmia
Cultius (Biologia)
Cultures (Biology)
Issue Date: 12-Mar-2019
Publisher: Frontiers Media
Abstract: Sepsis is a serious health condition worldwide, affecting more than 30 million people globally each year. Blood culture (BC) is generally used to diagnose sepsis because of the low quantity of microbes occurring in the blood during such infections. However, ~50% of bloodstream infections (BSI) give negative BC, this figure being higher for sepsis, which delays the start of appropriate antimicrobial therapy. This prospective study evaluated a multiplex real-time polymerase chain reaction, the MagicplexTM Sepsis test (MP), for the detection of pathogens from whole blood, comparing it to routine BC. We analyzed 809 blood samples from 636 adult patients, with 132/809 (16.3%) of the samples positive for one or more relevant microorganism according to BC and/or MP. The sensitivity and specificity of MP were 29 and 95%, respectively, while the level of agreement between BC and MP was 87%. The rate of contaminated samples was higher for BC (10%) than MP (4.8%) (P < 0.001). Patients with only MP-positive samples were more likely to be on antimicrobial treatment (47%) than those with only BC-positive samples (18%) (P = 0.002). In summary, the MP test could be useful in some clinical setting, such as among patients on antibiotic therapy. Nevertheless, a low sensitivity demonstrated impairs its use as a part of a routine diagnostic algorithm.
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It is part of: Frontiers in Cellular and Infection Microbiology, 2019, vol. 9, p. 56
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ISSN: 2235-2988
Appears in Collections:Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Fonaments Clínics)
Articles publicats en revistes (ISGlobal)

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