Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/137901
Title: Optimization of dissolution rate measurements of poor-soluble drugs
Other Titles: Optimització de les mesures de velocitat de dissolució de fàrmacs amb baixa solubilitat
Author: Garcia Marmol, Cristina
Director/Tutor: Fuguet i Jordà, Elisabet
Ràfols Llach, Clara
Keywords: Medicaments
Solubilitat
Cromatografia de líquids d'alta resolució
Treballs de fi de grau
Drugs
Solubility
High performance liquid chromatography
Bachelor's thesis
Issue Date: Jun-2019
Abstract: One of the most important parameters to be determined during the drug development process is the dissolution rate. The in vitro study of this property is essential to evaluate the bioavailability of drugs in the drug discovery process. Traditional methods for dissolution rate determination require high amounts of the active pharmaceutical ingredient. However, it is a problem when drug characterization is carried out in the early stages of the process, when only little amounts of drug are available. For these reasons, miniaturized methods, which use low drug amounts, were developed. The gastrointestinal dissolution method is an example of miniaturized method, and it is used in the present work. The fact of extracting part of solution at several times, for off-line quantification is not a problem in traditional methods, because it does not affect the dissolution rate profile curve, as the extracted volume is not significant compared to the total one. However, that might not be the case in miniaturized methods, where smaller dissolution volumes are used. In order to compare the effect of extracting part of solution to quantify off-line the concentration of drug on the profile curve, dissolution rate measurements have been carried out for 120 minutes at constant pH for two different drugs: trazodone and warfarin. First, dissolution profiles have been determined in situ, with spectrophotometric detection. Next, experiments have been repeated performing several extractions and quantifying off-line by HPLC. Results show that dissolution profiles are comparable, although the solubility of the compounds can be a limiting factor. Determined profiles are similar in the case of trazodone. However, warfarin, with a lower solubility than trazodone, needs support from a cosolvent to reach equivalent concentrations in the HPLC profile.
Note: Treballs Finals de Grau de Química, Facultat de Química, Universitat de Barcelona, Any: 2019, Tutores: Elisabet Fuguet Jordà, Clara Ràfols Llach
URI: http://hdl.handle.net/2445/137901
Appears in Collections:Treballs Finals de Grau (TFG) - Química

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