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Title: NCS-1 associates with adenosine A(2A) receptors and modulates receptor function
Author: Navarro Brugal, Gemma
Hradsky, Johannes
Lluís i Biset, Carme
Casadó, Vicent
McCormick, Peter
Kreutz, Michael R.
Mikhaylova, Marina
Keywords: Adenosina
Proteïnes G
G Proteins
Issue Date: 18-Apr-2012
Publisher: Frontiers Media
Abstract: Modulation of G protein-coupled receptor (GPCR) signaling by local changes in intracellular calcium concentration is an established function of Calmodulin (CaM) which is known to interact with many GPCRs. Less is known about the functional role of the closely related neuronal EF-hand Ca2+-sensor proteins that frequently associate with CaM targets with different functional outcome. In the present study we aimed to investigate if a target of CaM the A2A adenosine receptor is able to associate with two other neuronal calcium binding proteins (nCaBPs), namely NCS-1 and caldendrin. Using bioluminescence resonance energy transfer (BRET) and co-immunoprecipitation experiments we show the existence of A2A NCS-1 complexes in living cells whereas caldendrin did not associate with A2A receptors under the conditions tested. Interestingly, NCS-1 binding modulated downstream A2A receptor intracellular signaling in a Ca2+-dependent manner. Taken together this study provides further evidence that neuronal Ca2+-sensor proteins play an important role in modulation of GPCR signaling.
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It is part of: Frontiers In Molecular Neuroscience, 2012, vol. 5, num. 53, p. 1-10
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ISSN: 1662-5099
Appears in Collections:Articles publicats en revistes (Bioquímica i Fisiologia)
Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

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