Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/138638
Title: Role of the pre-neck appendage protein (DpoSep7) from phage vB_SepiS-phiIPLA7 in biofilm disruption of staphylococcal species
Author: Gutiérrez, Diana
Briers, Yves
Rodríguez Rubio, Lorena
Martínez, Beatriz
Rodríguez, Ana
Lavigne, Rob
García, Pilar
Keywords: Estafilococs
Staphylococcus
Issue Date: 25-Nov-2015
Publisher: Frontiers Media
Abstract: Staphylococcus epidermidis and Staphylococcus aureus are important causative agents of hospital-acquired infections and bacteremia, likely due to their ability to form biofilms. The production of a dense exopolysaccharide (EPS) matrix enclosing the cells slows the penetration of antibiotic down, resulting in therapy failure. The EPS depolymerase (Dpo7) derived from bacteriophage vB_SepiS-phiIPLA7, was overexpressed in Escherichia coli and characterized. A dose dependent but time independent response was observed after treatment of staphylococcal 24 h-biofilms with Dpo7. Maximum removal (>90%) of biofilm-attached cells was obtained with 0.15 μM of Dpo7 in all polysaccharide producer strains but Dpo7 failed to eliminate polysaccharide-independent biofilm formed by S. aureus V329. Moreover, the pre-treatment of polystyrene surfaces with Dpo7 reduced the biofilm biomass by 53-85% in the 67% of the tested strains. This study supports the use of phage-encoded EPS depolymerases to prevent and disperse staphylococcal biofilms, thereby making bacteria more susceptible to the action of antimicrobials.
Note: Reproducció del document publicat a: https://doi.org/10.3389/fmicb.2015.01315
It is part of: Frontiers in Microbiology, 2015, vol. 6, p. 1315
URI: http://hdl.handle.net/2445/138638
Related resource: https://doi.org/10.3389/fmicb.2015.01315
ISSN: 1664-302X
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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