Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/138857
Title: Elusive role of the CD94/NKG2C NK cell receptor in the response to cytomegalovirus: novel experimental observations in a reporter cell system
Author: Pupuleku, Aldi
Costa García, Marcel
Farré Marimon, Domènec
Hengel, Hartmut
Angulo Aguado, Ana
Muntasell, Aura
López Botet, Miguel
Keywords: Citomegalovirus
Citometria de fluxe
Cytomegaloviruses
Flow cytometry
Issue Date: 24-Oct-2017
Publisher: Frontiers Media
Abstract: Human cytomegalovirus (HCMV) infection promotes the differentiation and persistent expansion of a mature NK cell subset, which displays high surface levels of the activating CD94/NKG2C NK cell receptor, together with additional distinctive phenotypic and functional features. The mechanisms underlying the development of adaptive NK cells remain uncertain but some observations support the involvement of a cognate interaction of CD94/NKG2C with ligand(s) displayed by HCMV-infected cells. To approach this issue, the heterodimer and its adaptor (DAP12) were expressed in the human Jurkat leukemia T cell line; signaling was detected by transfection of a reporter plasmid encoding for Luciferase (Luc) under NFAT/AP1-dependent control. Engagement of the receptor by solid-phase bound CD94- or NKG2C-specific monoclonal antibodies (mAbs) triggered Luc expression. Moreover, reporter activation was detectable upon interaction with HLA-E+ 721.221 (.221-AEH) cells, as well as with 721.221 cells incubated with synthetic peptides, which stabilized surface expression of endogenous HLA-E; the response was specifically antagonized by soluble NKG2C- and HLA-E-specific mAbs. By contrast, activation of Jurkat-NKG2C+ was undetectable upon interaction with Human Fetal Foreskin Fibroblasts (HFFF) infected with HCMV laboratory strains (i.e., AD169, Towne), regardless of their differential ability to preserve surface HLA-E expression. On the other hand, infection with two clinical isolates or with the endotheliotropic TB40/E strain triggered Jurkat-NKG2C+ activation; yet, this response was not inhibited by blocking mAbs and was independent of CD94/NKG2C expression. The results are discussed in the framework of previous observations supporting the hypothetical existence of specific ligand(s) for CD94/NKG2C in HCMV-infected cells.
Note: Reproducció del document publicat a: https://doi.org/10.3389/fimmu.2017.01317
It is part of: Frontiers in Immunology, 2017, vol. 8
URI: http://hdl.handle.net/2445/138857
Related resource: https://doi.org/10.3389/fimmu.2017.01317
ISSN: 1664-3224
Appears in Collections:Articles publicats en revistes (Biomedicina)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

Files in This Item:
File Description SizeFormat 
677229.pdf2.12 MBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons