Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/138988
Title: Self-organized intestinal epithelial monolayers in crypt and villus-like domains show effective barrier function
Author: Altay, Gizem
Larrañaga, Enara
Tosi, Sebástien
Barriga, Francisco, M.
Batlle, Eduard
Fernández-Majada, Vanesa
Martínez, Elena
Keywords: Cèl·lules epitelials
Intestins
Epithelial cells
Intestines
Issue Date: 12-Jul-2019
Publisher: Springer Nature Publishing
Abstract: Intestinal organoids have emerged as a powerful in vitro tool for studying intestinal biology due to their resemblance to in vivo tissue at the structural and functional levels. However, their sphere-like geometry prevents access to the apical side of the epithelium, making them unsuitable for standard functional assays designed for flat cell monolayers. Here, we describe a simple method for the formation of epithelial monolayers that recapitulates the in vivo-like cell type composition and organization and that is suitable for functional tissue barrier assays. In our approach, epithelial monolayer spreading is driven by the substrate stiffness, while tissue barrier function is achieved by the basolateral delivery of medium enriched with stem cell niche and myofibroblast-derived factors. These monolayers contain major intestinal epithelial cell types organized into proliferating crypt-like domains and differentiated villus-like regions, closely resembling the in vivo cell distribution. As a unique characteristic, these epithelial monolayers form functional epithelial barriers with an accessible apical surface and physiologically relevant transepithelial electrical resistance values. Our technology offers an up-to-date and novel culture method for intestinal epithelium, providing an in vivo-like cell composition and distribution in a tissue culture format compatible with high-throughput drug absorption or microbe-epithelium interaction studies.
It is part of: Scientific Reports, 2019, vol. 9
URI: http://hdl.handle.net/2445/138988
Related resource: https://doi.org/10.1038/s41598-019-46497-x
Appears in Collections:Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
Articles publicats en revistes (Electrònica)

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