Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/139039
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | López Aladid, Rubén | - |
dc.contributor.author | Guiu, Alba | - |
dc.contributor.author | Mosquera, Maria Mar | - |
dc.contributor.author | López Medrano, Francisco | - |
dc.contributor.author | Cofán Pujol, Federico | - |
dc.contributor.author | Linares, Laura | - |
dc.contributor.author | Torre Cisneros, Julián | - |
dc.contributor.author | Vidal, Elisa | - |
dc.contributor.author | Moreno Camacho, Ma. Asunción | - |
dc.contributor.author | Aguado, José María | - |
dc.contributor.author | Cordero, Elisa | - |
dc.contributor.author | Martin Gandul, Cecilia | - |
dc.contributor.author | Carratalà, Jordi | - |
dc.contributor.author | Sabé, Nuria | - |
dc.contributor.author | Niubó, Jordi | - |
dc.contributor.author | Cervera, Carlos | - |
dc.contributor.author | Capón, Alicia | - |
dc.contributor.author | Cervilla, Anna | - |
dc.contributor.author | Santos, Marta | - |
dc.contributor.author | Bodro, Marta | - |
dc.contributor.author | Muñoz, Patricia | - |
dc.contributor.author | Farinas, Maria Carmen | - |
dc.contributor.author | Antón, Andrés | - |
dc.contributor.author | Aranzamendi, Maitane | - |
dc.contributor.author | Montejo, Miguel | - |
dc.contributor.author | Pérez Romero, Pilar | - |
dc.contributor.author | Len, Óscar | - |
dc.contributor.author | Marcos, Ma. Angeles | - |
dc.date.accessioned | 2019-09-02T10:55:46Z | - |
dc.date.available | 2019-09-02T10:55:46Z | - |
dc.date.issued | 2019-07-18 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2445/139039 | - |
dc.description.abstract | Objectives: The aim of this study was to identify CMV drug resistance mutations (DRM) in solid organ transplant (SOT) recipients with suspected resistance comparing next-generation sequencing (NGS) with Sanger sequencing and assessing risk factors and the clinical impact of resistance. Methods: Using Sanger sequencing as the reference method, we prospectively assessed the ability of NGS to detect CMV DRM in the UL97 and UL54 genes in a nationwide observational study from September 2013 to August 2016. Results: Among 44 patients recruited, 14 DRM were detected by Sanger in 12 patients (27%) and 20 DRM were detected by NGS, in 16 (36%). NGS confirmed all the DRM detected by Sanger. The additional six mutations detected by NGS were present in <20% of the sequenced population, being located in the UL97 gene and conferring high-level resistance to ganciclovir. The presence of DRM by NGS was associated with lung transplantation (p = 0.050), the administration of prophylaxis (p = 0.039), a higher mean time between transplantation and suspicion of resistance (p = 0.038) and longer antiviral treatment duration before suspicion (p = 0.024). However, the latter was the only factor independently associated with the presence of DRM by NGS in the multivariate analysis (OR 2.24, 95% CI 1.03 to 4.87). Conclusions: NGS showed a higher yield than Sanger sequencing for detecting CMV resistance mutations in SOT recipients. The presence of DRM detected by NGS was independently associated with longer antiviral treatment. | - |
dc.format.extent | 13 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0219701 | - |
dc.relation.ispartof | PLoS ONE, 2019, vol. 14 , num. 7, p. e0219701 | - |
dc.relation.uri | http://dx.doi.org/10.1371/journal.pone.0219701 | - |
dc.rights | cc by (c) López Aladid et al., 2019 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | - |
dc.source | Articles publicats en revistes (ISGlobal) | - |
dc.subject.classification | Citomegalovirus | - |
dc.subject.classification | Trasplantament hepàtic | - |
dc.subject.other | Cytomegaloviruses | - |
dc.subject.other | Hepatic transplantation | - |
dc.title | Improvement in detecting cytomegalovirus drug resistance mutations in solid organ transplant recipients with suspected resistance using next generation sequencing | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2019-08-02T18:00:48Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 31318908 | - |
Appears in Collections: | Articles publicats en revistes (ISGlobal) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Lopez-AladidR_PLoS_One_2019.pdf | 512.67 kB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License