Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/139400
Title: Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells
Author: Thottacherry, Joseph J.
Kosmalska, Anita J.
Kumar, Amit
Vishen, Amit S.
Elosegui-Artola, Alberto
Pradhan, Susav
Sharma, Sumit
Singh, Parvinder P.
Guadamillas, Marta C.
Chaudhary, Natasha
Vishwakarma, Ram
Trepat Guixer, Xavier
Pozo, Miguel A. del
Parton, Robert G.
Rao, Madan
Pullarkat, Pramod
Roca-Cusachs Soulere, Pere
Mayor, Satyajit
Keywords: Biologia molecular
Transport biològic
Membranes (Biologia)
Molecular biology
Biological transport
Membranes (Biology)
Issue Date: 11-Oct-2018
Publisher: Nature Publishing Group
Abstract: Plasma membrane tension regulates many key cellular processes. It is modulated by, and can modulate, membrane trafficking. However, the cellular pathway(s) involved in this interplay is poorly understood. Here we find that, among a number of endocytic processes operating simultaneously at the cell surface, a dynamin independent pathway, the CLIC/GEEC (CG) pathway, is rapidly and specifically upregulated upon a sudden reduction of tension. Moreover, inhibition (activation) of the CG pathway results in lower (higher) membrane tension. However, alteration in membrane tension does not directly modulate CG endocytosis. This requires vinculin, a mechano-transducer recruited to focal adhesion in adherent cells. Vinculin acts by controlling the levels of a key regulator of the CG pathway, GBF1, at the plasma membrane. Thus, the CG pathway directly regulates membrane tension and is in turn controlled via a mechano-chemical feedback inhibition, potentially leading to homeostatic regulation of membrane tension in adherent cells.
Note: Reproducció del document publicat a: https://doi.org/10.1038/s41467-018-06738-5
It is part of: Nature Communications, 2018, vol. 9, p. 4217
URI: http://hdl.handle.net/2445/139400
Related resource: https://doi.org/10.1038/s41467-018-06738-5
ISSN: 2041-1723
Appears in Collections:Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
Articles publicats en revistes (Biomedicina)

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