Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/139403
Title: Molecular clutch drives cell response to surface viscosity
Author: Bennett, Mark
Cantini, Marco
Reboud, Julien
Cooper, Jonathan M.
Roca-Cusachs Soulere, Pere
Salmeron-Sanchez, Manuel
Keywords: Biologia molecular
Teixits (Histologia)
Viscositat
Molecular biology
Tissues
Viscosity
Issue Date: 6-Feb-2018
Publisher: National Academy of Sciences
Abstract: Cell response to matrix rigidity has been explained by the mechanical properties of the actin-talin-integrin-fibronectin clutch. Here the molecular clutch model is extended to account for cell interactions with purely viscous surfaces (i.e., without an elastic component). Supported lipid bilayers present an idealized and controllable system through which to study this concept. Using lipids of different diffusion coefficients, the mobility (i.e., surface viscosity) of the presented ligands (in this case RGD) was altered by an order of magnitude. Cell size and cytoskeletal organization were proportional to viscosity. Furthermore, there was a higher number of focal adhesions and a higher phosphorylation of FAK on less-mobile (more-viscous) surfaces. Actin retrograde flow, an indicator of the force exerted on surfaces, was also seen to be faster on more mobile surfaces. This has consequential effects on downstream molecules; the mechanosensitive YAP protein localized to the nucleus more on less-mobile (more-viscous) surfaces and differentiation of myoblast cells was enhanced on higher viscosity. This behavior was explained within the framework of the molecular clutch model, with lower viscosity leading to a low force loading rate, preventing the exposure of mechanosensitive proteins, and with a higher viscosity causing a higher force loading rate exposing these sites, activating downstream pathways. Consequently, the understanding of how viscosity (regardless of matrix stiffness) influences cell response adds a further tool to engineer materials that control cell behavior.
Note: Reproducció del document publicat a: https://doi.org/10.1073/pnas.1710653115
It is part of: Proceedings of the National Academy of Sciences of the United States of America - PNAS, 2018, vol. 115, num. 6, p. 1192-1197
URI: http://hdl.handle.net/2445/139403
Related resource: https://doi.org/10.1073/pnas.1710653115
ISSN: 0027-8424
Appears in Collections:Articles publicats en revistes (Biomedicina)
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))

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