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Title: Traction forces at the cytokinetic ring regulate cell division and polyploidy in the migrating zebrafish epicardium
Author: Uroz, Marina
Garcia Puig, Anna
Elosegui Artola, Alberto
Abenza, Juan F.
Marín Llauradó, Ariadna
Pujals Riatós, Silvia
Conte, Vito
Albertazzi, Lorenzo
Roca-Cusachs Soulere, Pere
Raya Chamorro, Ángel
Trepat Guixer, Xavier
Keywords: Divisió cel·lular
Cell division
Issue Date: 3-Jun-2019
Publisher: Springer Nature
Abstract: Epithelial repair and regeneration are driven by collective cell migration and division. Both cellular functions involve tightly controlled mechanical events, but how physical forces regulate cell division in migrating epithelia is largely unknown. Here we show that cells dividing in the migrating zebrafish epicardium exert large cell–extracellular matrix (ECM) forces during cytokinesis. These forces point towards the division axis and are exerted through focal adhesions that connect the cytokinetic ring to the underlying ECM. When subjected to high loading rates, these cytokinetic focal adhesions prevent closure of the contractile ring, leading to multi-nucleation through cytokinetic failure. By combining a clutch model with experiments on substrates of different rigidity, ECM composition and ligand density, we show that failed cytokinesis is triggered by adhesion reinforcement downstream of increased myosin density. The mechanical interaction between the cytokinetic ring and the ECM thus provides a mechanism for the regulation of cell division and polyploidy that may have implications in regeneration and cancer.
Note: Versió postprint del document publicat a:
It is part of: Nature Materials, 2019, vol. 18
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Appears in Collections:Articles publicats en revistes (Biomedicina)
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
Publicacions de projectes de recerca finançats per la UE

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