Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/139906
Title: Ghrelin and its receptors in Gglthead Sea bream: nutritional regulation
Author: Perelló Amorós, Miquel
Vélez, Emilio J.
Vela Albesa, Jaume
Sánchez-Moya, Albert
Riera Heredia, Natàlia
Heden, Ida
Fernández Borrás, J. (Jaume)
Blasco Mínguez, Josefina
Calduch Giner, Josep Àlvar
Navarro Álvarez, Isabel
Capilla Campos, Encarnación
Jönsson Bergman, Elisabeth
Pérez Sánchez, Jaume
Gutiérrez Fruitós, Joaquín
Keywords: Hormones
Alimentació animal
Orada
Hormones
Animal feeding
Sparus aurata
Issue Date: 30-Jul-2018
Publisher: Frontiers Media
Abstract: Ghrelin is involved in the regulation of growth in vertebrates through controlling different functions, such as feed intake, metabolism, intestinal activity or growth hormone (Gh) secretion. The aim of this work was to identify the sequences of preproghrelin and Ghrelin receptors (ghsrs), and to study their responses to different nutritional conditions in gilthead sea bream (Sparus aurata) juveniles. The structure and phylogeny of S. aurata preproghrelin was analyzed, and a tissue screening was performed. The effects of 21 days of fasting and 2, 5, 24 h, and 7 days of refeeding on plasma levels of Ghrelin, Gh and Igf-1, and the gene expression of preproghrelin, ghsrs and members of the Gh/Igf-1 system were determined in key tissues. preproghrelin and the receptors are well conserved, being expressed mainly in stomach, and in the pituitary and brain, respectively. Twenty-one days of fasting resulted in a decrease in growth while Ghrelin plasma levels were elevated to decrease at 5 h post-prandial when pituitary ghsrs expression was minimum. Gh in plasma increased during fasting and slowly felt upon refeeding, while plasma Igf-1 showed an inverse profile. Pituitary gh expression augmented during fasting reaching maximum levels at 1 day post-feeding while liver igf-1 expression and that of its splice variants decreased to lowest levels. Liver Gh receptors expression was down-regulated during fasting and recovered after refeeding. This study demonstrates the important role of Ghrelin during fasting, its acute down-regulation in the post-prandial stage and its interaction with pituitary Ghsrs and Gh/Igf-1 axis.
Note: Reproducció del document publicat a: https://doi.org/10.3389/fendo.2018.00399
It is part of: Frontiers In Endocrinology, 2018, vol. 9, p. 399
URI: http://hdl.handle.net/2445/139906
Related resource: https://doi.org/10.3389/fendo.2018.00399
ISSN: 1664-2392
Appears in Collections:Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)

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