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Title: Differential expression of var subgroups and PfSir2a genes in afebrile Plasmodium falciparum malaria: a matched case-control study
Author: Gupta, Himanshu
Galatas, Beatriz
Matambisso, Gloria
Nhamussua, Lidia
Cisteró, Pau
Bassat Orellana, Quique
Casellas, Aina
Macete, Eusebio Víctor
Aponte, John J.
Sacoor, Charfudin
Alonso, Pedro
Saúte, Francisco
Guinovart, Caterina
Aide, Pedro Carlos Paulino
Mayor Aparicio, Alfredo Gabriel
Keywords: Malària
Issue Date: 23-Sep-2019
Publisher: BioMed Central
Abstract: Background: Poor knowledge on the afebrile Plasmodium falciparum biology limits elimination approaches to target asymptomatic malaria. Therefore, the association of parasite factors involved in cytoadhesion, parasite multiplication and gametocyte maturation with afebrile malaria was assessed. Methods: Plasmodium falciparum isolates were collected from febrile (axillary temperature≥37.5 °C or a reported fever in the previous 24 h) and afebrile (fever neither at the visit nor in the previous 24 h) individuals residing in Southern Mozambique. var, PfSir2a and Pfs25 transcript levels were determined by reverse transcriptase quantitative PCRs (RT-qPCRs) and compared among 61 pairs of isolates matched by parasite density, age and year of sample collection. Results: The level of varC and PfSir2a transcripts was higher in P. falciparum isolates from afebrile individuals (P≤0.006), while varB and DC8 genes (P≤0.002) were higher in isolates from individuals with febrile infections. After adjusting the analysis by area of residence, doubling the relative transcript unit (RTU) of varC and PfSir2a was associated with a 29.7 (95% CI 4.6–192.3) and 8.5 (95% CI 1.9–32.2) fold increases, respectively, of the odds of being afebrile. In contrast, doubling the RTU of varB and DC8 was associated with a 0.8 (95% CI 0.05–0.6) and 0.2 (95% CI 0.04–0.6) fold changes, respectively, of the odds of being afebrile. No signifcant diferences were found for Pfs25 transcript levels in P. falciparum isolates from afebrile and febrile individuals. Conclusions: var and gametocyte-specifc transcript patterns in febrile and afebrile infections from southern Mozambique matched by age, parasite density and recruitment period suggest similar transmissibility but diferential expression of variant antigens involved in cytoadhesion and immune-evasion.
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It is part of: Malaria Journal, 2019, vol. 18
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ISSN: 1475-2875
Appears in Collections:Articles publicats en revistes (ISGlobal)

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