Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/141528
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dc.contributor.authorSuh, Hoonkyo-
dc.contributor.authorZhou, Qi-Gang-
dc.contributor.authorFernandez-Carasa, Irene-
dc.contributor.authorClemenson Jr., Gregory D.-
dc.contributor.authorPons-Espinal, Meritxell-
dc.contributor.authorJeoung Ro, Eun-
dc.contributor.authorMarti, Mercè-
dc.contributor.authorRaya Chamorro, Ángel-
dc.contributor.authorGage, Fred H.-
dc.contributor.authorConsiglio, Antonella-
dc.date.accessioned2019-10-02T15:04:08Z-
dc.date.available2019-10-02T15:04:08Z-
dc.date.issued2018-11-15-
dc.identifier.issn1662-5099-
dc.identifier.urihttp://hdl.handle.net/2445/141528-
dc.description.abstractUsing a lentivirus-mediated labeling method, we investigated whether the adult hippocampus retains long-lasting, self-renewing neural stem cells (NSCs). We first showed that a single injection of a lentiviral vector expressing a green fluorescent protein (LV PGK-GFP) into the subgranular zone (SGZ) of the adult hippocampus enabled an efficient, robust, and long-term marking of self-renewing NSCs and their progeny. Interestingly, a subset of labeled cells showed the ability to proliferate multiple times and give rise to Sox2+ cells, clearly suggesting the ability of NSCs to self-renew for an extensive period of time (up to 6 months). In addition, using GFP+ cells isolated from the SGZ of mice that received a LV PGK-GFP injection 3 months earlier, we demonstrated that some GFP+ cells displayed the essential properties of NSCs, such as self-renewal and multipotency. Furthermore, we investigated the plasticity of NSCs in a perforant path transection, which has been shown to induce astrocyte formation in the molecular layer of the hippocampus. Our lentivirus (LV)-mediated labeling study revealed that hippocampal NSCs are not responsible for the burst of astrocyte formation, suggesting that signals released from the injured perforant path did not influence NSC fate determination. Therefore, our studies showed that a gene delivery system using LVs is a unique method to be used for understanding the complex nature of NSCs and may have translational impact in gene therapy by efficiently targeting NSCs.-
dc.format.extent10 p.-
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherFrontiers Media-
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.3389/fnmol.2018.00415-
dc.relation.ispartofFrontiers In Molecular Neuroscience, 2018, vol. 11, p. 415-
dc.relation.urihttps://doi.org/10.3389/fnmol.2018.00415-
dc.rightscc-by (c) Suh, Hoonkyo et al., 2018-
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es-
dc.sourceArticles publicats en revistes (Patologia i Terapèutica Experimental)-
dc.subject.classificationLesions cerebrals-
dc.subject.classificationCèl·lules mare-
dc.subject.classificationDianes farmacològiques-
dc.subject.otherBrain damage-
dc.subject.otherStem cells-
dc.subject.otherDrug targeting-
dc.titleLong-term labeling of hippocampal neural stem cells by a lentiviral vector-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.identifier.idgrec687540-
dc.date.updated2019-10-02T15:04:08Z-
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/311736/EU//PD-HUMMODEL-
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess-
dc.identifier.pmid30498432-
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)
Publicacions de projectes de recerca finançats per la UE
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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