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Title: Proteins in human brain cortex are modified by oxidation, glycoxidation, and lipoxidation. Effects of alzheimer disease and identification of lipoxidation targets
Author: Pamplona, Reinald
Dalfó Capella, Esther
Ayala, Victòria
Bellmunt, Maria Josep
Prat Corominas, Joan
Ferrer, Isidro (Ferrer Abizanda)
Portero Otin, Manuel
Keywords: Malalties cerebrals
Malaltia d'Alzheimer
Brain diseases
Alzheimer's disease
Issue Date: 3-Jun-2005
Publisher: American Society for Biochemistry and Molecular Biology
Abstract: Diverse oxidative pathways, such as direct oxidation of amino acids, glycoxidation, and lipoxidation could contribute to Alzheimer disease pathogenesis. A global survey for the amount of structurally characterized probes for these reactions is lacking and could overcome the lack of specificity derived from measurement of 2,4-dinitrophenylhydrazine reactive carbonyls. Consequently we analyzed (i) the presence and concentrations of glutamic and aminoadipic semialdehydes, N(epsilon)-(carboxymethyl)-lysine, N(epsilon)-(carboxyethyl)-lysine, and N(epsilon)-(malondialdehyde)-lysine by means of gas chromatography/mass spectrometry, (ii) the biological response through expression of the receptor for advanced glycation end products, (iii) the fatty acid composition in brain samples from Alzheimer disease patients and age-matched controls, and (iv) the targets of N(epsilon)-(malondialdehyde)-lysine formation in brain cortex by proteomic techniques. Alzheimer disease was associated with significant, although heterogeneous, increases in the concentrations of all evaluated markers. Alzheimer disease samples presented increases in expression of the receptor for advanced glycation end products with high molecular heterogeneity. Samples from Alzheimer disease patients also showed content of docosahexaenoic acid, which increased lipid peroxidizability. In accordance, N(epsilon)-(malondialdehyde)-lysine formation targeted important proteins for both glial and neuronal homeostasis such as neurofilament L, alpha-tubulin, glial fibrillary acidic protein, ubiquinol-cytochrome c reductase complex protein I, and the beta chain of ATP synthase. These data support an important role for lipid peroxidation-derived protein modifications in Alzheimer disease pathogenesis.
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It is part of: Journal of Biological Chemistry, 2005, vol. 280, num. 22, p. 21522-21530
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ISSN: 0021-9258
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)

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