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http://hdl.handle.net/2445/143335
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DC Field | Value | Language |
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dc.contributor.author | Ràfols Llach, Clara | - |
dc.contributor.author | Amézqueta, Susana | - |
dc.contributor.author | Fuguet i Jordà, Elisabet | - |
dc.contributor.author | Bosch, Elisabeth | - |
dc.date.accessioned | 2019-10-29T12:39:40Z | - |
dc.date.available | 2019-12-18T06:10:22Z | - |
dc.date.issued | 2017-12-18 | - |
dc.identifier.issn | 0731-7085 | - |
dc.identifier.uri | http://hdl.handle.net/2445/143335 | - |
dc.description.abstract | Interaction thermodynamics between warfarin, a very popular anticoagulant, and Sudlow I binding siteof human (HSA) or bovine (BSA) serum albumin have been examined in strictly controlled experimentalconditions (HEPES buffer 50 mM, pH 7.4 and 25◦C) by means of isothermal titration calorimetry (ITC),fluorescence spectrometry (FS) and frontal analysis capillary electrophoresis (FA/CE). Each technique isbased on measurements of a different property of the biochemical system, and then the results allow acritical discussion about the suitability of each approach to estimate the drug-protein binding parameters.The strongest interaction step is properly evaluated by the three assayed approaches being the derivedbinding constants strongly consistent: from 4 × 104to 7 × 104for HSA and from 0.8 × 105to 1.2 × 105forBSA. Binding enthalpy variations also show consistent results: −5.4 and −5.6 Kcal/mol for HSA and −4.3and −3.7 Kcal/mol for BSA, as measured by ITC and FS, respectively. Further high order interaction eventsfor both albumins are detected only by FA/CE. | - |
dc.format.extent | 8 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1016/j.jpba.2017.12.008 | - |
dc.relation.ispartof | Journal of Pharmaceutical and Biomedical Analysis, 2017, vol. 150, p. 452-459 | - |
dc.relation.uri | https://doi.org/10.1016/j.jpba.2017.12.008 | - |
dc.rights | cc-by-nc-nd (c) Elsevier B.V., 2017 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es | - |
dc.source | Articles publicats en revistes (Enginyeria Química i Química Analítica) | - |
dc.subject.classification | Anticoagulants (Medicina) | - |
dc.subject.classification | Electroforesi capil·lar | - |
dc.subject.other | Anticoagulants (Medicine) | - |
dc.subject.other | Capillary electrophoresis | - |
dc.title | Molecular interactions between warfarin and human (HSA) or bovine(BSA) serum albumin evaluated by isothermal titration calorimetry(ITC), fluorescence spectrometry (FS) and frontal analysis capillaryelectrophoresis (FA/CE) | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 675473 | - |
dc.date.updated | 2019-10-29T12:39:40Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
Files in This Item:
File | Description | Size | Format | |
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675473.pdf | 1.28 MB | Adobe PDF | View/Open |
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