Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/145441
Title: | Microbiota analysis of biofilms on experimental abutments mimicking dental implants: an in vivo model |
Author: | Cortés Acha, Berta Barbosa de Figueiredo, Rui Pedro Seminago, Ramon Roig, Francisco J. Llorens, Carlos Valmaseda Castellón, Eduardo |
Keywords: | Biofilms Implants dentals Microbiologia Biofilms Dental implants Microbiology |
Issue Date: | 1-Oct-2017 |
Publisher: | American Academy of Periodontology |
Abstract: | Background: the microbiota colonizing dental implants has been said to be similar to the microbiome surrounding teeth. In the absence of inflammation, a biofilm with pathologic bacteria can cover implant surfaces exposed to the oral cavity, for example, due to a remodeling process. The aim of the present study is to identify microbiota surrounding exposed dental implants in patients with and without a history of periodontitis through a deep-sequencing approach. Methods: an experimental abutment with the same surface and structure as a commercially available dental implant was used. Bacterial DNA was isolated, and the 16S ribosomal RNA gene was amplified and sequenced. Multiplexed tag-encoded sequencing of DNA from the samples was performed, and the reads were processed by metagenomic rapid annotation.Results: a wide variety of bacteria, 96 species, were identified. The most frequently found bacteria were Fusobacterium nucleatum and Prevotella denticola. Some species generally associated with periodontitis were found to a greater extent in patients without a history of periodontitis. Some bacteria that have never been described as part of the oral microbiome were identified in the present sample.Conclusions: analysis of data suggests that the bacteria surrounding exposed dental implants form a diverse microbiome regardless of the periodontal profile of patients. Further research is needed to clarify the role of these microorganisms in the oral environment. |
Note: | Reproducció del document publicat a: https://doi.org/10.1902/jop.2017.170051 |
It is part of: | Journal of Periodontology, 2017, vol. 88, num. 10, p. 1090-1104 |
URI: | http://hdl.handle.net/2445/145441 |
Related resource: | https://doi.org/10.1902/jop.2017.170051 |
ISSN: | 0022-3492 |
Appears in Collections: | Articles publicats en revistes (Odontoestomatologia) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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675195.pdf | 2.81 MB | Adobe PDF | View/Open |
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