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|Title:||Microbiota analysis of biofilms on experimental abutments mimicking dental implants: an in vivo model|
|Author:||Cortés Acha, Berta|
Roig, Francisco J.
Valmaseda Castellón, Eduardo
|Publisher:||American Academy of Periodontology|
|Abstract:||Background: the microbiota colonizing dental implants has been said to be similar to the microbiome surrounding teeth. In the absence of inflammation, a biofilm with pathologic bacteria can cover implant surfaces exposed to the oral cavity, for example, due to a remodeling process. The aim of the present study is to identify microbiota surrounding exposed dental implants in patients with and without a history of periodontitis through a deep-sequencing approach. Methods: an experimental abutment with the same surface and structure as a commercially available dental implant was used. Bacterial DNA was isolated, and the 16S ribosomal RNA gene was amplified and sequenced. Multiplexed tag-encoded sequencing of DNA from the samples was performed, and the reads were processed by metagenomic rapid annotation.Results: a wide variety of bacteria, 96 species, were identified. The most frequently found bacteria were Fusobacterium nucleatum and Prevotella denticola. Some species generally associated with periodontitis were found to a greater extent in patients without a history of periodontitis. Some bacteria that have never been described as part of the oral microbiome were identified in the present sample.Conclusions: analysis of data suggests that the bacteria surrounding exposed dental implants form a diverse microbiome regardless of the periodontal profile of patients. Further research is needed to clarify the role of these microorganisms in the oral environment.|
|Note:||Reproducció del document publicat a: https://doi.org/10.1902/jop.2017.170051|
|It is part of:||Journal of Periodontology, 2017, vol. 88, num. 10, p. 1090-1104|
|Appears in Collections:||Articles publicats en revistes (Odontoestomatologia)|
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
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