Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/146859
Title: Novel association of high c-reactive protein levels and A69S at risk alleles in wet age-related macular degeneration women.
Author: Fernandez Robredo, Patricia
Recalde Maestre, Sergio
Hernandez, Maria
Zarranz Ventura, Javier
Molins Monteys, Blanca
Casaroli Marano, Ricardo Pedro
Adán Civera, Alfredo
Saenz de Viteri, Manuel
Garcia Layana, Alfredo
Keywords: Diferències entre sexes
Malalties de la retina
Degeneració (Patologia)
Sex differences
Retinal diseases
Degeneration (Pathology)
Issue Date: 14-Aug-2018
Publisher: Frontiers Media
Abstract: Purpose: To explore the relationship between plasma C-reactive protein (CRP) levels, the main ARMS2 gene single nucleotide polymorphism (SNP), and gender in patients with neovascular age-related macular degeneration (wet AMD). Methods: Our study included 131 patients with wetAMD [age-related eye disease study (AREDS) category 4] and 153 control participants (AREDS category 1) from two Spanish retinal units. CRP levels were determined on blood samples by high-sensitivity ELISA assay. According to their CRP level, subjects were categorized into three well-established CRP categories: low (< 1.00 mg/L, L-CRP), moderate (1-2.99 mg/L, M-CRP), and high (> 3.00 mg/L, H-CRP). Genomic DNA was extracted from oral swabs using QIAcube (Qiagen, Hilden, Germany) and the A69S; rs10490924 of ARMS2 gene was genotyped by allelic discrimination with validated TaqMan assays (Applied Biosystems, Foster City, CA, USA). Univariate and multivariate logistic regression adjusted for age was used to analyze the genomic frequencies and to calculate odds ratio (OR) using SNPStats software. Results: Considering CRP risk categories, H-CRP group showed a significant [OR 4.0 (1.9-8.3)] association with wetAMD compared to L-CRP group. The risk genotypes of A69S (TT) SNPs showed an association with wetAMD risk [OR 14.0 (4.8-40.8)]. Interestingly, the gender stratification of the CRP categories showed a significant increase in CRP levels in wetAMD women compared with control women [OR 6.9 (2.2-22.3)] and with wetAMD men [OR 4.6 (1.3-16.9)]. In addition, the subgroup analysis of CRP within A69S genotype and gender showed a link in women between the A69S and CRP levels in the AMD group compared to controls [OR 4.2 (1.4-12.6)]. Conclusion: Our study shows, for the first time, that a different genetic association related with gender could contribute to AMD risk. As a consequence, the risk of female gender in the different CRP levels and A69S SNP frequencies could be taken into consideration to the established risk relationship of high levels of CRP and its association with risk A69S genotype.
Note: Reproducció del document publicat a: https://doi.org/10.3389/fimmu.2018.01862
It is part of: Frontiers in Immunology, 2018, vol. 9
URI: http://hdl.handle.net/2445/146859
Related resource: https://doi.org/10.3389/fimmu.2018.01862
ISSN: 1664-3224
Appears in Collections:Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

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