Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/147228
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dc.contributor.authorGarcía, Juan Ignacio-
dc.contributor.authorKelley, Holden V.-
dc.contributor.authorMeléndez, Johanna-
dc.contributor.authorAlvarez de León, Rosa Alejandra-
dc.contributor.authorCastillo, Alejandra-
dc.contributor.authorSidiki, Sabeen-
dc.contributor.authorYusoof, Kizil A.-
dc.contributor.authorNunes, Elizabete-
dc.contributor.authorLópez Téllez, Cesar-
dc.contributor.authorMejía Villatoro, Carlos Rodolfo-
dc.contributor.authorIkeda, Janet-
dc.contributor.authorGarcía-Basteiro, Alberto L.-
dc.contributor.authorWang, Shu-Hua-
dc.contributor.authorTorrelles, Jordi B.-
dc.date.accessioned2020-01-08T14:31:21Z-
dc.date.available2020-01-08T14:31:21Z-
dc.date.issued2019-11-29-
dc.identifier.issn2045-2322-
dc.identifier.urihttp://hdl.handle.net/2445/147228-
dc.description.abstractTuberculosis (TB) disease still kills 1-person every 21-seconds. Few TB diagnostic tests are considered truly appropriate for point of care settings. The WHO-endorsed immunodiagnostic Alere Determine Lipoarabinomannan Ag-test (LAM-test) detects Mycobacterium tuberculosis complex LAM in urine, and its use is recommended for TB diagnosis among HIV co-infected individuals with low CD4 T-cell counts. Here we found that a simple 15-minute enzymatic treatment at room temperature of LAM-spiked urine with \xCE\xB1-mannosidase (for human TB), and LAM-spiked milk with combined lactase and caseinase (for bovine TB), enhanced 10-fold the detection levels of the LAM-test and thus, improved the detection of LAM by the LAM-test in urine and milk that otherwise could be missed in the field. Future separate clinical research studies specifically designed to address the potential of these findings are required.-
dc.format.extent6 p.-
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherNature Publishing Group-
dc.relation.isformatofReproducció del document publicat a: http://dx.doi.org/10.1038/s41598-019-54537-9-
dc.relation.ispartofScientific Reports, 2019, vol. 9, p. 18012-
dc.relation.urihttp://dx.doi.org/10.1038/s41598-019-54537-9-
dc.rightscc by (c) García et al., 2019-
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/-
dc.subject.classificationTuberculosi-
dc.subject.classificationDiagnòstic immunològic-
dc.subject.otherTuberculosis-
dc.subject.otherImmunodiagnosis-
dc.titleImproved Alere Determine Lipoarabinomannan Antigen Detection Test for the Diagnosis of Human and Bovine Tuberculosis by Manipulating Urine and Milk-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.date.updated2019-12-20T19:01:33Z-
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess-
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