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http://hdl.handle.net/2445/147807
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DC Field | Value | Language |
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dc.contributor.author | Castella, Maria | - |
dc.contributor.author | Boronat, Anna | - |
dc.contributor.author | Martín Ibáñez, Raquel | - |
dc.contributor.author | Rodríguez, Vanina | - |
dc.contributor.author | Suñé, Guillermo | - |
dc.contributor.author | Caballero, Miguel | - |
dc.contributor.author | Marzal Martí, Berta | - |
dc.contributor.author | Pérez-Amill, Lorena | - |
dc.contributor.author | Martín-Antonio, Beatriz | - |
dc.contributor.author | Castaño, Julio | - |
dc.contributor.author | Bueno, Clara | - |
dc.contributor.author | Balagué, Olga | - |
dc.contributor.author | González-Navarro, Europa Azucena | - |
dc.contributor.author | Serra Pagès, Carles | - |
dc.contributor.author | Engel Rocamora, Pablo | - |
dc.contributor.author | Vilella, Ramon | - |
dc.contributor.author | Benítez-Ribas, Daniel | - |
dc.contributor.author | Ortiz-Maldonado Gibson, Valentín | - |
dc.contributor.author | Cid Vidal, Joan | - |
dc.contributor.author | Tabera, Jaime | - |
dc.contributor.author | Canals i Coll, Josep M. | - |
dc.contributor.author | Lozano, Miquel | - |
dc.contributor.author | Baumann, Tycho | - |
dc.contributor.author | Vilarrodona, Anna | - |
dc.contributor.author | Trias, Esteve | - |
dc.contributor.author | Campo Güerri, Elias | - |
dc.contributor.author | Menéndez Buján, Pablo | - |
dc.contributor.author | Urbano Ispizua, Álvaro | - |
dc.contributor.author | Yagüe, Jordi | - |
dc.contributor.author | Pérez Galán, Patricia | - |
dc.contributor.author | Rives, Susana | - |
dc.contributor.author | Delgado, Julio (Delgado González) | - |
dc.contributor.author | Juan, Manel | - |
dc.date.accessioned | 2020-01-14T16:39:59Z | - |
dc.date.available | 2020-01-14T16:39:59Z | - |
dc.date.issued | 2018-12-06 | - |
dc.identifier.issn | 2329-0501 | - |
dc.identifier.uri | http://hdl.handle.net/2445/147807 | - |
dc.description.abstract | Genetically modifying autologous T cells to express an anti-CD19 chimeric antigen receptor (CAR) has shown impressive response rates for the treatment of CD19+ B cell malignancies in several clinical trials (CTs). Making this treatment available to our patients prompted us to develop a novel CART19 based on our own anti-CD19 antibody (A3B1), followed by CD8 hinge and transmembrane region, 4-1BB- and CD3z-signaling domains. We show that A3B1 CAR T cells are highly cytotoxic and specific against CD19+ cells in vitro, inducing secretion of pro-inflammatory cytokines and CAR T cell proliferation. In vivo, A3B1 CAR T cells are able to fully control disease progression in an NOD.Cg-Prkdcscid Il2rdtm1Wjl/SzJ (NSG) xenograph B-ALL mouse model. Based on the pre-clinical data, we conclude that our CART19 is clearly functional against CD19+ cells, to a level similar to other CAR19s currently being used in the clinic. Concurrently, we describe the implementation of our CAR T cell production system, using lentiviral vector and CliniMACS Prodigy, within a medium-sized academic institution. The results of the validation phase show our system is robust and reproducible, while maintaining a low cost that is affordable for academic institutions. Our model can serve as a paradigm for similar institutions, and it may help to make CAR T cell treatment available to all patients. | - |
dc.format.extent | 11 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Cell Press | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1016/j.omtm.2018.11.010 | - |
dc.relation.ispartof | Molecular Therapy-Methods & Clinical Development, 2018, vol. 12, p. 134-144 | - |
dc.relation.uri | https://doi.org/10.1016/j.omtm.2018.11.010 | - |
dc.rights | cc-by (c) Castella, Maria et al., 2018 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Medicina) | - |
dc.subject.classification | Immunoteràpia | - |
dc.subject.classification | Leucèmia | - |
dc.subject.classification | Limfomes | - |
dc.subject.classification | Cèl·lules T | - |
dc.subject.other | Immunotheraphy | - |
dc.subject.other | Leukemia | - |
dc.subject.other | Lymphomas | - |
dc.subject.other | T cells | - |
dc.title | Development of a novel anti-CD19 chimeric antigen receptor: A paradigm for an affordable CAR T cell production at academic institutions | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 684889 | - |
dc.date.updated | 2020-01-14T16:40:00Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/646903/EU//INFANTLEUKEMIA | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/811220/EU//IT4B-ALL | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.idimarina | 4148378 | - |
dc.identifier.pmid | 30623002 | - |
Appears in Collections: | Articles publicats en revistes (Medicina) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
Files in This Item:
File | Description | Size | Format | |
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684889.pdf | 2.03 MB | Adobe PDF | View/Open |
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