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Title: Differential expression of long non-coding RNAs are related to proliferation and histological diversity in follicular lymphomas
Author: Roisman, Alejandro
Castellano, Giancarlo
Navarro, Alba
González Farré, Blanca
Pérez Galán, Patricia
Esteve Codina, Anna
Dabad, Marc
Heath, Simon C.
Gut, Marta
Bosio, Mattia
Bellot, Pau
Salembier, Philippe
Oliveras, Albert
Slavutsky, Irma
Magnano, Laura
Horn, Heike
Rosenwald, Andreas
Ott, German
Aymerich Gregorio, Marta
López Guillermo, Armando
Jares Gerboles, Pedro
Martín-Subero, José Ignacio
Campo Güerri, Elias
Hernández, Luis
Keywords: Limfomes
Proliferació cel·lular
Cell proliferation
Issue Date: Feb-2019
Publisher: John Wiley & Sons
Abstract: Long non‐coding RNAs (lncRNAs) comprise a family of non‐coding transcripts that are emerging as relevant gene expression regulators of different processes, including tumour development. To determine the possible contribution of lncRNA to the pathogenesis of follicular lymphoma (FL) we performed RNA‐sequencing at high depth sequencing in primary FL samples ranging from grade 1‐3A to aggressive grade 3B variants using unpurified (n = 16) and purified (n = 12) tumour cell suspensions from nodal samples. FL grade 3B had a significantly higher number of differentially expressed lncRNAs (dif‐lncRNAs) with potential target coding genes related to cell cycle regulation. Nine out of the 18 selected dif‐lncRNAs were validated by quantitative real time polymerase chain reaction in an independent series (n = 43) of FL. RP4‐694A7.2 was identified as the top deregulated lncRNA potentially involved in cell proliferation. RP4‐694A7.2 silencing in the WSU‐FSCCL FL cell line reduced cell proliferation due to a block in the G1/S phase. The relationship between RP4‐694A7.2 and proliferation was confirmed in primary samples as its expression levels positively related to the Ki‐67 proliferation index. In summary, lncRNAs are differentially expressed across the clinico‐biological spectrum of FL and a subset of them, related to cell cycle, may participate in cell proliferation regulation in these tumours.
Note: Versió postprint del document publicat a:
It is part of: British Journal of Haematology, 2019, vol. 184, num. 3, p. 373-383
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ISSN: 0007-1048
Appears in Collections:Articles publicats en revistes (Fonaments Clínics)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

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