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Title: Aspirin induces cell death and caspase-dependent phosphatidylserine externalization in HT-29 human colon adenocarcinoma cells
Author: Castaño Boldú, Esther
Dalmau, Mireia
Barragán, Montse
Pueyo, Gloria
Bartrons Bach, Ramon
Gil i Santano, Joan
Keywords: Apoptosi
Efectes secundaris dels medicaments
Drug side effects
Issue Date: 1-Sep-1999
Publisher: Cancer Research UK
Abstract: The induction of cell death by aspirin was analysed in HT-29 colon carcinoma cells. Aspirin induced two hallmarks of apoptosis: nuclear chromatin condensation and increase in phosphatidylserine externalization. However, aspirin did not induce either oligonucleosomal fragmentation of DNA, decrease in DNA content or nuclear fragmentation. The effect of aspirin on Annexin V binding was inhibited by the caspase inhibitor Z-VAD.fmk, indicating the involvement of caspases in the apoptotic action of aspirin. However, aspirin did not induce proteolysis of PARP, suggesting that aspirin does not increase nuclear caspase 3-like activity in HT-29 cells. This finding may be related with the 'atypical' features of aspirin-induced apoptosis in HT-29 cells.
Note: Versió postprint del document publicat a:
It is part of: British Journal of Cancer, 1999, vol. 81, num. 2, p. 294-299
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ISSN: 0007-0920
Appears in Collections:Articles publicats en revistes (Ciències Fisiològiques)

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