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Title: Structural insights into “acid blobs and negative noodles” – The androgen receptor as a case study
Author: Szulc, Elzbieta Maria
Director/Tutor: Salvatella i Giralt, Xavier
Keywords: Factors de transcripció
Càncer de pròstata
Transcription factors
Prostate cancer
Issue Date: 9-Jan-2020
Publisher: Universitat de Barcelona
Abstract: [eng] Androgen receptor is a transcription factor that plays a key role in prostate cancer development and progression. Its N-terminal intrinsically disordered activation domain is an example of ill- structured negatively charged "acid blob" or "negative noodle" described in 1988 by Paul B. Sigler. In his model the transcription pre-initiation complex formation relies on non-specific interactions between the "negative noodles" of the transcriptional activating proteins and hydroxyl groups of the heptapeptide repeats of the C-terminal domain in the largest subunit of RNA polymerase II. Here, we take advantage of various biophysical methods to understand the AR activation domain characteristics, its ability to undergo liquid-liquid phase separation and the importance of both for protein-protein interactions of the domain. We show relevance of the secondary structure elements in the AD function despite its general "acid blob" character.
Appears in Collections:Tesis Doctorals - Facultat - Farmàcia i Ciències de l'Alimentació

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