Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/149016
Title: Antibodies to inhibitory synaptic proteins in neurological syndromes associated with glutamic acid decarboxylase autoimmunity
Author: Gresa Arribas, Nuria
Ariño Rodríguez, Helena
Martínez Hernández, Eugenia
Petit Pedrol, Mar
Sabater, Lidia
Saiz Hinajeros, Albert
Dalmau Obrador, Josep
Graus Ribas, Francesc
Keywords: Epilèpsia
Neurologia
Epilepsy
Neurology
Issue Date: 16-Mar-2015
Publisher: Public Library of Science (PLoS)
Abstract: Antibodies to glutamic acid decarboxylase (GAD-ab) associate to different neurological syndromes. It is unknown if the diversity in syndrome association represents epitopes in different immunodominant domains or co-existence of antibodies to other proteins of the inhibitory synapsis. We examined the serum and CSF of 106 patients with anti-GAD related syndromes (39 cerebellar ataxia, 32 stiff-person syndrome [SPS], 18 epilepsy, and 17 limbic encephalitis [LE]). GAD65-ab titres were quantified by ELISA. Immunoblot was used to determine if the antibody-targeted epitopes of GAD65 and GAD67 were linear. A cell-based assay (CBA) with HEK293 cells expressing the GAD65 N-terminal, central catalytic domain, or C-terminal was used to investigate the immunodominant domains. Antibodies to GAD67, gamma-aminobutyric acid A receptor (GABAaR), glycine receptor (GlyR), GABAaR-associated protein (GABARAP), and gephyrin were determined with CBA. GAD-ab internalization was investigated using cultured rat hippocampal neurons. CSF GAD65-ab titres were higher in patients with cerebellar ataxia and LE compared to those with SPS (p = 0.02). GAD67-ab were identified in 81% of sera and 100% of CSF. GAD65-ab recognized linear epitopes in 98% of the patients and GAD67-ab in 42% (p<0.001). The GAD65 catalytic domain was recognized by 93% of sera, and the three domains by 22% of sera and 74% of CSF (p<0.001). Six patients had GABAaR-ab and another 6 had GlyR-ab without association to distinctive symptoms. None of the patients had gephyrin- or GABARAP-ab. GAD65-ab were not internalized by live neurons. Overall, these findings show that regardless of the neurological syndrome, the CSF immune response against GAD is more widespread than that of the serum and that there is no specific association between clinical phenotype and the presence of antibodies against other proteins of the inhibitory synapsis.
Note: Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0121364
It is part of: PLoS One, 2015, vol. 10, num. 3, p. e0121364
URI: http://hdl.handle.net/2445/149016
Related resource: https://doi.org/10.1371/journal.pone.0121364
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Medicina)

Files in This Item:
File Description SizeFormat 
690249.pdf5.25 MBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons