Please use this identifier to cite or link to this item:
Title: Osteoblastic cell response on high-rough titanium coatings by cold spray
Author: Martín Vilardell, Anna
Cinca i Luis, Núria
Garcia Giralt, Natàlia
Dosta Parras, Sergi
García Cano, Irene
Nogués Solán, Xavier
Guilemany, J. M. (José María)
Keywords: Titani
Proliferació cel·lular
Fecundació in vitro
Cell proliferation
Fertilization in vitro
Issue Date: 1-Jun-2018
Publisher: Chapman & Hall
Abstract: Highly rough and porous commercially pure titanium coatings have been directly produced for first time by the cold spray technology, which is a promising technology in front of the vacuum plasma spray for oxygen sensitive materials. The wettability properties as well as the biocompatibility evaluation have been compared to a simply sand blasted Ti6Al4V alloy substrate. Surface topographies were analysed using confocal microscopy. Next, osteoblast morphology (Phalloidin staining), proliferation (MTS assay), and differentiation (alkaline phosphatase activity) were examined along 1, 7 and 14 days of cell culture on the different surfaces. Finally, mineralization by alizarin red staining was quantified at 28 days of cell culture. The contact angle values showed an increased hydrophilic behaviour on the as-sprayed surface with a good correlation to the biological response. A higher cell viability, proliferation and differentiation were obtained for highly rough commercial pure titanium coatings in comparison with sand blasted substrates. Cell morphology was similar in all coatings tested; at 14 days both samples showed extended filopodia. A higher amount of calcium-rich deposits was detected on highly rough surfaces. In summary, in-vitro results showed an increase of biological properties when surface roughness increases.
Note: Versió postprint del document publicat a:
It is part of: Journal of Materials Science-Materials in Medicine, 2018, vol. 29, num. 2
Related resource:
ISSN: 0957-4530
Appears in Collections:Articles publicats en revistes (Ciència dels Materials i Química Física)

Files in This Item:
File Description SizeFormat 
679757.pdf312.96 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.