Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/149364
Title: | Osteoblastic cell response on high-rough titanium coatings by cold spray |
Author: | Martín Vilardell, Anna Cinca i Luis, Núria Garcia Giralt, Natàlia Dosta Parras, Sergi García Cano, Irene Nogués Solán, Xavier Guilemany, J. M. (José María) |
Keywords: | Titani Proliferació cel·lular Fecundació in vitro Titanium Cell proliferation Fertilization in vitro |
Issue Date: | 1-Jun-2018 |
Publisher: | Chapman & Hall |
Abstract: | Highly rough and porous commercially pure titanium coatings have been directly produced for first time by the cold spray technology, which is a promising technology in front of the vacuum plasma spray for oxygen sensitive materials. The wettability properties as well as the biocompatibility evaluation have been compared to a simply sand blasted Ti6Al4V alloy substrate. Surface topographies were analysed using confocal microscopy. Next, osteoblast morphology (Phalloidin staining), proliferation (MTS assay), and differentiation (alkaline phosphatase activity) were examined along 1, 7 and 14 days of cell culture on the different surfaces. Finally, mineralization by alizarin red staining was quantified at 28 days of cell culture. The contact angle values showed an increased hydrophilic behaviour on the as-sprayed surface with a good correlation to the biological response. A higher cell viability, proliferation and differentiation were obtained for highly rough commercial pure titanium coatings in comparison with sand blasted substrates. Cell morphology was similar in all coatings tested; at 14 days both samples showed extended filopodia. A higher amount of calcium-rich deposits was detected on highly rough surfaces. In summary, in-vitro results showed an increase of biological properties when surface roughness increases. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1007/s10856-018-6026-8 |
It is part of: | Journal of Materials Science-Materials in Medicine, 2018, vol. 29, num. 2 |
URI: | http://hdl.handle.net/2445/149364 |
Related resource: | https://doi.org/10.1007/s10856-018-6026-8 |
ISSN: | 0957-4530 |
Appears in Collections: | Articles publicats en revistes (Ciència dels Materials i Química Física) |
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