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Title: PrionW: a server to identify proteins containing glutamine/asparagine rich prion-like domains and their amyloid cores
Author: Zambrano, Rafael
Conchillo Sole, Oscar
Iglesias, Valentín
Illa Pujagut, Ricard
Rousseau, Frederic
Schymkowitz, Joost
Sabaté Lagunas, Raimon
Daura, Xavier
Ventura, Salvador
Keywords: Malalties per prions
Prion diseases
Issue Date: 1-Jul-2015
Publisher: Oxford University Press
Abstract: Prions are a particular type of amyloids with the ability to self-perpetuate and propagate in vivo. Prionlike conversion underlies important biological processes but is also connected to human disease. Yeast prions are the best understood transmissible amyloids. In these proteins, prion formation from an initially soluble state involves a structural conversion, driven, in many cases, by specific domains enriched in glutamine/asparagine (Q/N) residues. Importantly, domains sharing this compositional bias are also present in the proteomes of higher organisms, thus suggesting that prion-like conversion might be an evolutionary conserved mechanism. We have recently shown that the identification and evaluation of the potency of amyloid nucleating sequences in putative prion domains allows discrimination of genuine prions. PrionW is a web application that exploits this principle to scan sequences in order to identify proteins containing Q/N enriched prion-like domains (PrLDs) in large datasets. When used to scan the complete yeast proteome, PrionW identifies previously experimentally validated prions with high accuracy. Users can analyze up to 10 000 sequences at a time, PrLD-containing proteins are identified and their putative PrLDs and amyloid nucleating cores visualized and scored. The output files can be downloaded for further analysis. PrionW server can be accessed at
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It is part of: Nucleic Acids Research, 2015, vol. 43, num. W1, p. W331-W337
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ISSN: 0305-1048
Appears in Collections:Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica)
Articles publicats en revistes (Institut de Nanociència i Nanotecnologia (IN2UB))

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