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Title: Quantification of malaria antigens PfHRP2 and pLDH by quantitative suspension array technology in whole blood, dried blood spot and plasma
Author: Martiáñez Vendrell, Xavier
Jiménez, Alfons
Vásquez, Ana
Campillo, Ana
Incardona, Sandra
González, Raquel
Gamboa, Dionicia
Torres, Katherine
Oyibo, Wellington
Faye, Babacar
Macete, Eusebio Víctor
Menéndez, Clara
Ding, Xavier C.
Mayor Aparicio, Alfredo Gabriel
Keywords: Malària
Plasma sanguini
Blood plasma
Issue Date: 9-Jan-2020
Publisher: BioMed Central
Abstract: Background: Malaria diagnostics by rapid diagnostic test (RDT) relies primarily on the qualitative detection of Plasmodium falciparum histidine-rich protein 2 (PfHRP2) and Plasmodium spp lactate dehydrogenase (pLDH). As novel RDTs with increased sensitivity are being developed and implemented as point of care diagnostics, highly sensitive laboratory-based assays are needed for evaluating RDT performance. Here, a quantitative suspension array technol‑ ogy (qSAT) was developed, validated and applied for the simultaneous detection of PfHRP2 and pLDH in a variety of biological samples (whole blood, plasma and dried blood spots) from individuals living in diferent endemic countries. Results: The qSAT was specifc for the target antigens, with analytical ranges of 6.8 to 762.8 pg/ml for PfHRP2 and 78.1 to 17076.6 pg/ml for P. falciparum LDH (Pf-LDH). The assay detected Plasmodium vivax LDH (Pv-LDH) at a lower sensitivity than Pf-LDH (analytical range of 1093.20 to 187288.5 pg/ml). Both PfHRP2 and pLDH levels determined using the qSAT showed to positively correlate with parasite densities determined by quantitative PCR (Spearman r=0.59 and 0.75, respectively) as well as microscopy (Spearman r=0.40 and 0.75, respectively), suggesting the assay to be a good predictor of parasite density. Conclusion: This immunoassay can be used as a reference test for the detection and quantifcation of PfHRP2 and pLDH, and could serve for external validation of RDT performance, to determine antigen persistence after parasite clearance, as well as a complementary tool to assess malaria burden in endemic settings.
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It is part of: Malaria Journal, 2020, vol. 19
Related resource:
ISSN: 1475-2875
Appears in Collections:Articles publicats en revistes (ISGlobal)

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