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http://hdl.handle.net/2445/150434
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DC Field | Value | Language |
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dc.contributor.author | Martiáñez Vendrell, Xavier | - |
dc.contributor.author | Jiménez, Alfons | - |
dc.contributor.author | Vásquez, Ana | - |
dc.contributor.author | Campillo, Ana | - |
dc.contributor.author | Incardona, Sandra | - |
dc.contributor.author | González, Raquel | - |
dc.contributor.author | Gamboa, Dionicia | - |
dc.contributor.author | Torres, Katherine | - |
dc.contributor.author | Oyibo, Wellington | - |
dc.contributor.author | Faye, Babacar | - |
dc.contributor.author | Macete, Eusebio Víctor | - |
dc.contributor.author | Menéndez, Clara | - |
dc.contributor.author | Ding, Xavier C. | - |
dc.contributor.author | Mayor Aparicio, Alfredo Gabriel | - |
dc.date.accessioned | 2020-02-17T14:21:36Z | - |
dc.date.available | 2020-02-17T14:21:36Z | - |
dc.date.issued | 2020-01-09 | - |
dc.identifier.issn | 1475-2875 | - |
dc.identifier.uri | http://hdl.handle.net/2445/150434 | - |
dc.description.abstract | Background: Malaria diagnostics by rapid diagnostic test (RDT) relies primarily on the qualitative detection of Plasmodium falciparum histidine-rich protein 2 (PfHRP2) and Plasmodium spp lactate dehydrogenase (pLDH). As novel RDTs with increased sensitivity are being developed and implemented as point of care diagnostics, highly sensitive laboratory-based assays are needed for evaluating RDT performance. Here, a quantitative suspension array technol‑ ogy (qSAT) was developed, validated and applied for the simultaneous detection of PfHRP2 and pLDH in a variety of biological samples (whole blood, plasma and dried blood spots) from individuals living in diferent endemic countries. Results: The qSAT was specifc for the target antigens, with analytical ranges of 6.8 to 762.8 pg/ml for PfHRP2 and 78.1 to 17076.6 pg/ml for P. falciparum LDH (Pf-LDH). The assay detected Plasmodium vivax LDH (Pv-LDH) at a lower sensitivity than Pf-LDH (analytical range of 1093.20 to 187288.5 pg/ml). Both PfHRP2 and pLDH levels determined using the qSAT showed to positively correlate with parasite densities determined by quantitative PCR (Spearman r=0.59 and 0.75, respectively) as well as microscopy (Spearman r=0.40 and 0.75, respectively), suggesting the assay to be a good predictor of parasite density. Conclusion: This immunoassay can be used as a reference test for the detection and quantifcation of PfHRP2 and pLDH, and could serve for external validation of RDT performance, to determine antigen persistence after parasite clearance, as well as a complementary tool to assess malaria burden in endemic settings. | - |
dc.format.extent | 11 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | BioMed Central | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1186/s12936-019-3083-5 | - |
dc.relation.ispartof | Malaria Journal, 2020, vol. 19 | - |
dc.relation.uri | http://dx.doi.org/10.1186/s12936-019-3083-5 | - |
dc.rights | cc by (c) Martiáñez Vendrell et al., 2020 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | - |
dc.source | Articles publicats en revistes (ISGlobal) | - |
dc.subject.classification | Malària | - |
dc.subject.classification | Plasma sanguini | - |
dc.subject.other | Malaria | - |
dc.subject.other | Blood plasma | - |
dc.title | Quantification of malaria antigens PfHRP2 and pLDH by quantitative suspension array technology in whole blood, dried blood spot and plasma | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2020-02-14T19:01:17Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 31918718 | - |
Appears in Collections: | Articles publicats en revistes (ISGlobal) |
Files in This Item:
File | Description | Size | Format | |
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Martia_ez-VendrellX_MalarJ_2020.pdf | 1.13 MB | Adobe PDF | View/Open |
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