Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/151387
Title: Improving separation optimization in capillary electrophoresis by using a general quality criterion
Author: Peró Gascón, Roger
Tascon, Marcos
Sanz Nebot, María Victoria
Gagliardi, Riccardo
Benavente Moreno, Fernando J. (Julián)
Keywords: Electroforesi capil·lar
Concentració dels ions d'hidrogen
Capillary electrophoresis
Hydrogen-ion concentration
Issue Date: 2020
Publisher: Elsevier B.V.
Abstract: In this paper we extend the use of the quality criterion t' to optimize separations in capillary electrophoresis (CE). The theoretical parameter t' takes into account not only the relative separation between a given pair of compounds but also their separation from the neutral species migrating with the electroosmotic flow (EOF). Furthermore, it can be composed for complex mixtures as a global multicriterium optimization function T', for a rapid, simple and reliable selection of optimized separation conditions by mathematical maximization. Here, we demonstrate the applicability of T' using as a variable the electrophoretic mobility (me) for the optimization of pH in the separation of a mixture of amyloid beta (Aβ) peptide fragments. In addition, it is shown the versatility of T' using other variables related to me, which do not require experimental measurements. This is the case with ionizable compounds as the Aβ peptide fragments, whose charge-to-mass ratios can be calculated if accurate pKa values are available in the literature. The excellent performance of T' for Aβ peptide fragments is further validated optimizing the pH for the separation of mixtures of harmala alkaloids (HAlks) and quinolone antibiotics.
Note: Versió postprint del document publicat a: https://doi.org/10.1016/j.talanta.2019.120399
It is part of: Talanta, 2020, vol. 208, p. 120399
URI: http://hdl.handle.net/2445/151387
Related resource: https://doi.org/10.1016/j.talanta.2019.120399
ISSN: 0039-9140
Appears in Collections:Articles publicats en revistes (Enginyeria Química i Química Analítica)

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