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Title: Identification of antihypertensive peptides in nutraceuticals by capillary electrophoresis-mass spectrometry
Author: Benavente Moreno, Fernando J. (Julián)
Peró Gascón, Roger
Pont Villanueva, Laura
Jaumot Soler, Joaquim
Barbosa Torralbo, José
Sanz Nebot, María Victoria
Keywords: Pèptids
Electroforesi capil·lar
Espectrometria de masses
Capillary electrophoresis
Mass spectrometry
Issue Date: 2018
Publisher: Elsevier B.V.
Abstract: We present capillary electrophoresis-mass spectrometry (CE-MS) in combination with advanced chemometric tools for the analysis of bioactive compounds in food, in particular for the identification of antihypertensive peptides in a nutraceutical derived from a bovine milk protein hydrolysate. Different extracts of the nutraceutical were analyzed by CE-MS, and the electropherograms were processed using a novel data analysis workflow that included regions of interest (ROIs) compression and multivariate curve resolution alternating least squares (MCR-ALS). MCR-ALS permitted the description of the nutraceutical extract as ten characteristic components with their electrophoretic profiles and mass spectra. Twenty-two compounds were tentatively identified as antihypertensive bovine casein fragments through a mass search in a database of bioactive peptides. The identity of 17 antihypertensive peptides was reliably confirmed by capillary electrophoresis-tandem mass spectrometry. The proposed analytical approach demonstrated the potential to obtain a characteristic and activity-related fingerprint for quality control and authentication of the antihypertensive nutraceutical.
Note: Versió postprint del document publicat a:
It is part of: Journal of Chromatography A, 2018, vol. 1579, p. 129-137
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ISSN: 0021-9673
Appears in Collections:Articles publicats en revistes (Enginyeria Química i Química Analítica)

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