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Title: The Effect of Small Cosolutes that Mimic Molecular Crowding Conditions on the Stability of Triplexes Involving Duplex DNA
Author: Aviñó Andrés, Anna
Mazzini, Stefania
Gargallo Gómez, Raimundo
Eritja i Casadellà, Ramon
Keywords: Oligonucleòtids
Issue Date: 5-Feb-2016
Publisher: MDPI
Abstract: Triplex stability is studied in crowding conditions using small cosolutes (ethanol, acetonitrile and dimethylsulfoxide) by ultraviolet (UV), circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopies. The results indicate that the triplex is formed preferentially when the triplex forming oligonucleotide (TFO) is RNA. In addition, DNA triplexes (D:D·D) are clearly less stable in cosolute solutions while the stability of the RNA triplexes (R:D·D) is only slightly decreased. The kinetic of triplex formation with RNA-TFO is slower than with DNA-TFO and the thermal stability of the triplex is increased with the salt concentration in EtOH-water solutions. Accordingly, RNA could be considered a potential molecule to form a stable triplex for regulatory purposes in molecular crowding conditions.
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It is part of: International Journal of Molecular Sciences, 2016, vol. 17, num. 2, p. 211
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ISSN: 1661-6596
Appears in Collections:Articles publicats en revistes (Enginyeria Química i Química Analítica)

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