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http://hdl.handle.net/2445/154184
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DC Field | Value | Language |
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dc.contributor.author | Vu Cantero, Diem-Lan | - |
dc.contributor.author | Bosch, Albert | - |
dc.contributor.author | Pintó Solé, Rosa María | - |
dc.contributor.author | Ribes Mora, Enric | - |
dc.contributor.author | Guix Arnau, Susana | - |
dc.date.accessioned | 2020-03-27T08:39:47Z | - |
dc.date.available | 2020-03-27T08:39:47Z | - |
dc.date.issued | 2019-06-14 | - |
dc.identifier.issn | 0022-538X | - |
dc.identifier.uri | http://hdl.handle.net/2445/154184 | - |
dc.description.abstract | MLB astroviruses were identified 10 years ago in feces from children with gastroenteritis of unknown etiology and have been unexpectedly detected in severe cases of meningitis/encephalitis, febrile illness of unknown etiology, and respiratory syndromes. The aim of this study was to establish a cell culture system supporting MLB astrovirus replication. We used two clinical strains to infect several cell lines, an MLB1 strain from a gastroenteritis case, and an MLB2 strain associated with a neurologic infection. Efforts to propagate the viruses in the Caco-2 cell line were unsuccessful. In contrast, we identified two human nonintestinal cell lines, Huh-7 and A549, permissive for both genotypes. After serial passages in the Huh-7.5 cell line, the adapted strains were able to establish persistent infections in the Huh-7.5, Huh-7AI, and A549 cell lines, with high viral loads (up to 10 log10 genome copies/ml) detected by quantitative reverse transcription-PCR (RT-qPCR) in the culture supernatant. Immunofluorescence assays demonstrated infection in about 10% of cells in persistently infected cultures. Electron microscopy revealed particles of 32 to 33 nm in diameter after negative staining of cell supernatants and capsid arrays in ultrathin sections with a particularly high production in Huh-7.5 cells. Interferon (IFN) expression by infected cells and effect of exogenous IFN varied depending on the type of infection and the cell line. The availability of a cell culture system to propagate MLB astroviruses represents a key step to better understand their replicative cycle, as well as a source of viruses to conduct a wide variety of basic virologic studies | - |
dc.format.extent | 18 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Society for Microbiology | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1128/JVI.00557-19 | - |
dc.relation.ispartof | Journal of Virology, 2019, vol. 93, num. 13 | - |
dc.relation.uri | https://doi.org/10.1128/JVI.00557-19 | - |
dc.rights | (c) American Society for Microbiology, 2019 | - |
dc.source | Articles publicats en revistes (Genètica, Microbiologia i Estadística) | - |
dc.subject.classification | Malalties intestinals | - |
dc.subject.classification | Virologia | - |
dc.subject.other | Intestinal diseases | - |
dc.subject.other | Virology | - |
dc.title | Human astrovirus MLB replication in vitro: persistence in extraintestinal cell lines | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 695112 | - |
dc.date.updated | 2020-03-27T08:39:48Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 31019055 | - |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
Files in This Item:
File | Description | Size | Format | |
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695112.pdf | 10.02 MB | Adobe PDF | View/Open |
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