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Title: Identification of Antimicrobial Peptides in Flowers of Sambucus Nigra
Author: Álvarez, Claudio Andrés
Barriga, Andrés
Albericio Palomera, Fernando
Romero, María Soledad
Guzmán, Fanny
Keywords: Medicina tradicional
Agents antiinfecciosos
Medicaments peptídics
Microorganismes patògens
Traditional medicine
Anti-infective agents
Influenza viruses
Peptide drugs
Pathogenic microorganisms
Issue Date: 27-May-2018
Publisher: MDPI
Abstract: The elder (Sambucus spp.) tree has a number of uses in traditional medicine. Previous studies have demonstrated the antimicrobial properties of elderberry liquid extract against human pathogenic bacteria and also influenza viruses. These properties have been mainly attributed to phenolic compounds. However, other plant defense molecules, such as antimicrobial peptides (AMPs), may be present. Here, we studied peptide extracts from flowers of Sambucus nigra L. The mass spectrometry analyses determined peptides of 3 to 3.6 kDa, among them, cysteine-rich peptides were identified with antimicrobial activity against various Gram-negative bacteria, including recurrent pathogens of Chilean aquaculture. In addition, membrane blebbing on the bacterial surface after exposure to the cyclotide was visualized by SEM microscopy and SYTOX Green permeabilization assay showed the ability to disrupt the bacterial membrane. We postulate that these peptides exert their action by destroying the bacterial membrane.
Note: Reproducció del document publicat a:
It is part of: Molecules, 2018, vol. 23, num. 5, p. 1033
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ISSN: 1420-3049
Appears in Collections:Articles publicats en revistes (Química Inorgànica i Orgànica)

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