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http://hdl.handle.net/2445/156118
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DC Field | Value | Language |
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dc.contributor.author | Kretzschmar, Anja | - |
dc.contributor.author | Schülke, Jan-Philip | - |
dc.contributor.author | Masana Nadal, Mercè | - |
dc.contributor.author | Dürre, Katharina | - |
dc.contributor.author | Müller, Marianne B. | - |
dc.contributor.author | Bausch, Andreas R. | - |
dc.contributor.author | Rein, Theo | - |
dc.date.accessioned | 2020-04-20T17:10:55Z | - |
dc.date.available | 2020-04-20T17:10:55Z | - |
dc.date.issued | 2018-12-11 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | http://hdl.handle.net/2445/156118 | - |
dc.description.abstract | Cytoskeletal dynamics are pivotal to memory, learning, and stress physiology, and thus psychiatric diseases. Downregulated in renal cell carcinoma 1 (DRR1) protein was characterized as the link between stress, actin dynamics, neuronal function, and cognition. To elucidate the underlying molecular mechanisms, we undertook a domain analysis of DRR1 and probed the effects on actin binding, polymerization, and bundling, as well as on actin-dependent cellular processes. METHODS: DRR1 domains were cloned and expressed as recombinant proteins to perform in vitro analysis of actin dynamics (binding, bundling, polymerization, and nucleation). Cellular actin-dependent processes were analyzed in transfected HeLa cells with fluorescence recovery after photobleaching (FRAP) and confocal microscopy. RESULTS: DRR1 features an actin binding site at each terminus, separated by a coiled coil domain. DRR1 enhances actin bundling, the cellular F-actin content, and serum response factor (SRF)-dependent transcription, while it diminishes actin filament elongation, cell spreading, and actin treadmilling. We also provide evidence for a nucleation effect of DRR1. Blocking of pointed end elongation by addition of profilin indicates DRR1 as a novel barbed end capping factor. CONCLUSIONS: DRR1 impacts actin dynamics in several ways with implications for cytoskeletal dynamics in stress physiology and pathophysiology. | - |
dc.format.extent | 30 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | MDPI | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3390/ijms19123993 | - |
dc.relation.ispartof | International Journal of Molecular Sciences, 2018, vol. 19, num. 12, p. 3993 | - |
dc.relation.uri | https://doi.org/10.3390/ijms19123993 | - |
dc.rights | cc-by (c) Kretzschmar, Anja et al., 2018 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Biomedicina) | - |
dc.subject.classification | Citosquelet | - |
dc.subject.classification | Proteïnes citosquelètiques | - |
dc.subject.other | Cytoskeleton | - |
dc.subject.other | Cytoskeletal proteins | - |
dc.title | The stress-inducible protein DRR1 exerts distinct effects on actin dynamics | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 683539 | - |
dc.date.updated | 2020-04-20T17:10:55Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 30545002 | - |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Biomedicina) |
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File | Description | Size | Format | |
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683539.pdf | 5.16 MB | Adobe PDF | View/Open |
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