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Title: Combined Use of the Ab105-2φΔCI Lytic Mutant Phage and Different Antibiotics in Clinical Isolates of Multi-Resistant Acinetobacter baumannii
Author: Blasco, Lucía
Ambroa, Antón
López, Maria
Fernández García, Laura
Bleriot, Ines
Trastoy Pena, Rocío
Ramos Vivas, José
Coenye, Tom
Fernández Cuenca, Felipe
Vila Estapé, Jordi
Martínez Martínez, Luis
Rodríguez Baño, Jesús
Pascual, Alvaro
Cisneros, Jose Miguel
Pachón, Jerónimo
Bou, Germán
Tomás, María
Keywords: Malalties bacterianes
Resistència als medicaments
Bacterial diseases
Drug resistance
Issue Date: 12-Nov-2019
Publisher: MDPI
Abstract: Phage therapy is an abandoned antimicrobial therapy that has been resumed in recent years. In this study, we mutated a lysogenic phage from Acinetobacter baumannii into a lytic phage (Ab105-2phi∆CI) that displayed antimicrobial activity against A. baumannii clinical strain Ab177_GEIH-2000 (isolated in the GEIH-REIPI Spanish Multicenter A. baumannii Study II 2000/2010, Umbrella Genbank Bioproject PRJNA422585, and for which meropenem and imipenem MICs of respectively, 32 μg/mL, and 16 μg/mL were obtained). We observed an in vitro synergistic antimicrobial effect (reduction of 4 log–7 log CFU/mL) between meropenem and the lytic phage in all combinations analyzed (Ab105-2phi∆CI mutant at 0.1, 1 and 10 MOI and meropenem at 1/4 and 1/8 MIC). Moreover, bacterial growth was reduced by 8 log CFU/mL for the combination of imipenem at 1/4 MIC plus lytic phage (Ab105-2phi∆CI mutant) and by 4 log CFU/mL for the combination of imipenem at 1/8 MIC plus lytic phage (Ab105-2phi∆CI mutant) at both MOI 1 and 10. These results were confirmed in an in vivo model (G. mellonella), and the combination of imipenem and mutant Ab105-2phi∆CI was most effective (p < 0.05). This approach could help to reduce the emergence of phage resistant bacteria and restore sensitivity to antibiotics used to combat multi-resistant strains of Acinetobacter baumannii.
Note: Reproducció del document publicat a: 10.3390/microorganisms7110556
It is part of: Microorganisms, 2019, vol. 7, num. 11, p. 556
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ISSN: 2076-2607
Appears in Collections:Articles publicats en revistes (Fonaments Clínics)
Articles publicats en revistes (ISGlobal)

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