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Title: Bacterial cellulose matrices to develop enzymatically active paper
Author: Buruaga Ramiro, Carolina
Valls Vidal, Cristina
Valenzuela Mayorga, Susana Valeria
Roncero, M. Blanca
Pastor Blasco, Francisco I. Javier
Díaz Lucea, Pilar
Martínez Martínez, Josefina
Keywords: Cel·lulosa
Issue Date: 31-Jan-2020
Publisher: Springer Verlag
Abstract: This work studies the suitability of bacterial cellulose (BC) matrices to prepare enzymatically active nanocomposites, in a framework of more environmentally friendly methodologies. After BC production and purification, two kind of matrices were obtained: BC in aqueous suspension and BC paper. A lipase was immobilised onto the BC matrices by physical adsorption, obtaining Lipase/BC nanocomposites. Neither morphology nor crystallinity, measured by scanning electron microscopy (SEM) and X-Ray diffractometry (XRD) respectively, of the BC were affected by the binding of the protein. The activity of Lipase/BC suspension and Lipase/BC paper was tested under different conditions, and the operational properties of the enzyme were evaluated. A shift towards higher temperatures, a broader pH activity range, and slight differences in the substrate preference were observed in the immobilised lipase, compared with the free enzyme. Specific activity was higher for Lipase/BC suspension (4.2 U/mg) than for Lipase/BC paper (1.7 U/mg) nanocomposites. However, Lipase/BC paper nanocomposites showed improved thermal stability, reusability, and durability. Enzyme immobilised onto BC paper retained 60% of its activity after 48 h at 60 ºC. It maintained 100% of the original activity after being recycled 10 times at pH 7 at 60 ºC and it remained active after being stored for more than a month at room temperature. The results suggested that lipase/BC nanocomposites are promising biomaterials for the development of green biotechnological devices with potential application in industrials bioprocesses of detergents and food industry and biomedicine. Lipase/BC paper nanocomposite might be a key component of bioactive paper for developing simple, handheld, and disposable devices.
Note: Versió postprint del document publicat a:
It is part of: Cellulose, 2020, vol. 27, p. 3413-3426
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ISSN: 0969-0239
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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