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Title: Enrichment of histidine containing peptides by on-line immobilized metal affinity solid-phase extraction capillary electrophoresis-mass spectrometry
Author: Peró Gascón, Roger
Giménez López, Estela
Sanz Nebot, María Victoria
Benavente Moreno, Fernando J. (Julián)
Keywords: Electroforesi capil·lar
Espectrometria de masses
Capillary electrophoresis
Mass spectrometry
Issue Date: 12-May-2020
Publisher: Elsevier B.V.
Abstract: The analysis and detection of targeted peptides has received due attention in many fields of proteomics research, such as discovery of biomarkers. In this regard, capillary electrophoresis-mass spectrometry (CE-MS) is widely used, however, direct analysis of low-abundance peptides such as histidine-containing peptides (His-peptides) in complex matrixes by CE-MS remains an analytical challenge. In the present study, an immobilised metal affinity solid-phase extraction containing Ni(II) coupled on-line to capillary electrophoresis-mass spectrometry (IMA-SPE-CE-MS) method has been developed to selectively enrich His-peptides from protein tryptic digests and enhance sensitivity. The method was optimised with α-casein and validated with other standard proteins (β-casein and κ-casein). Later, it was applied to an Escherichia Coli (E. Coli) whole cell lysate. IMA-SPE-CE-MS was very selective and allowed an enrichment factor up to 100-fold. The on-line enrichment and separation method coupled to MS detection is straightforward and advantageous over off-line pretreatment methods in terms of simplicity, cost-effectiveness and throughput.
Note: Versió postprint del document publicat a:
It is part of: Microchemical Journal, 2020, vol. 267, p. 121881
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ISSN: 0026-265X
Appears in Collections:Articles publicats en revistes (Enginyeria Química i Química Analítica)

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