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|Title:||Polymeric monolithic microcartridges with gold nanoparticles for the analysis of protein biomarkers by on-line solid-phase extraction capillary electrophoresis-mass spectrometry|
|Author:||Pont Villanueva, Laura|
Gagliardi, Leonardo G.
Sanz Nebot, María Victoria
Herrero-Martínez, J. M.
Benavente Moreno, Fernando J. (Julián)
Espectrometria de masses
|Abstract:||In this study, polymeric monoliths with gold nanoparticles (AuNP@monolith) were investigated as microcartridges for the analysis of protein biomarkers by on-line solid-phase extraction capillary electrophoresis-mass spectrometry (SPE-CE-MS). "Plug-and-play" microcartridges (7 mm) were prepared from a glycidyl methacrylate (GMA)-based monolithic capillary column (5 cm x 250 µm i.d.), which was modified with ammonia and subsequently functionalized with gold nanoparticles (AuNPs). The performance of these novel microcartridges was evaluated with human transthyretin (TTR), which is a protein related to different types of familial amyloidotic polyneuropathies (FAP). Protein retention depended on the isoelectric point of the protein (TTR pI~5.4) and elution was achieved with a basic phosphate solution. Under the optimized conditions, limits of detection (LODs) for TTR by AuNP@monolith-SPE-CE-MS were 50 times lower than by CE-MS (5 vs 250 mg L−1, with an ion trap (IT) mass spectrometer). The sensitivity enhancement was similar compared to SPE-CE-MS using immunoaffinity (IA) microcartridges with intact antibodies against TTR. Linearity, repeatability in migration times and peak areas, reusability, reproducibility and application to serum samples were also evaluated|
|Note:||Versió postprint del document publicat a: https://doi.org/10.1016/j.chroma.2020.461097|
|It is part of:||Journal of Chromatography A, 2020, vol. 1622, p. 461097-461097|
|Appears in Collections:||Articles publicats en revistes (Enginyeria Química i Química Analítica)|
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Document embargat fins el 18-4-2022
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