Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/162611
Title: Influence of pH and a porphyrin ligand on the stability of a G-quadruplex structure within a duplex segment near the promoter region of the SMARCA4 gene
Author: Navarro, Alba
Benabou, Sana
Eritja i Casadellà, Ramon
Gargallo Gómez, Raimundo
Keywords: Anàlisi multivariable
G-estructures
Porfirines
ADN
Multivariate analysis
G-structures
Porphyrins
DNA
Issue Date: 19-May-2020
Publisher: Elsevier B.V.
Abstract: In a previous work, the formation of G-quadruplex structures in a 44-nucleotide long sequence found near the promoter region of the SMARCA4 gene was reported. The central 25 nucleotides were able to fold into an antiparallel G-quadruplex structure, the stability of which was pH-dependent. In the present work, the effect of the presence of lateral nucleotides and the complementary cytosine-rich strand on the stability of this G-quadruplex has been characterized. Moreover, the role of the model ligand TMPyP4 has been studied. Spectroscopic and separation techniques, as well as multivariate data analysis methods, have been used with these purposes. The results have shown that stability of the G-quadruplex as a function of pH or temperature is greatly reduced in the presence of the lateral nucleotides. The influence of the complementary strand does not prevent the formation of the G-quadruplex. Moreover, attempts to modulate the equilibria by an external ligand led us to determine the influence of the TMPyP4 porphyrin on these complex equilibria. This study could eventually help to understand the regulation of SMARCA4 expression.
Note: Versió postprint del document publicat a: https://doi.org/10.1016/j.ijbiomac.2020.05.062
It is part of: International Journal of Biological Macromolecules, 2020, vol. 159, p. 383-393
URI: http://hdl.handle.net/2445/162611
Related resource: https://doi.org/10.1016/j.ijbiomac.2020.05.062
ISSN: 0141-8130
Appears in Collections:Articles publicats en revistes (Enginyeria Química i Química Analítica)

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