Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/162617
Title: Optimal environmental and culture conditions allow the in vitro coexistence of Pseudomonas aeruginosa and Staphylococcus aureus in stable biofilms
Author: Cendra Gascón, María del Mar
Blanco Cabra, Nuria
Pedraz López, Lucas
Torrents Serra, Eduard
Keywords: Malalties bacterianes
Microorganismes patògens
Injeccions
Bacterial diseases
Pathogenic microorganisms
Injections
Issue Date: 8-Nov-2019
Publisher: Nature Publishing Group
Abstract: The coexistence between species that occurs in some infections remains hard to achieve in vitro since bacterial fitness differences eventually lead to a single organism dominating the mixed culture. Pseudomonas aeruginosa and Staphylococcus aureus are major pathogens found growing together in biofilms in disease-affected lungs or wounds. Herein, we tested and analyzed different culture media, additives and environmental conditions to support P. aeruginosa and S. aureus coexistence in vitro. We have unraveled the potential of DMEM to support the growth of these two organisms in mature cocultured biofilms (three days old) in an environment that dampens the pH rise. Our conditions use equal initial inoculation ratios of both strains and allow the stable formation of separate S. aureus microcolonies that grow embedded in a P. aeruginosa biofilm, as well as S. aureus biofilm overgrowth when bovine serum albumin is added to the system. Remarkably, we also found that S. aureus survival is strictly dependent on a well-characterized phenomenon of oxygen stratification present in the coculture biofilm. An analysis of differential tolerance to gentamicin and ciprofloxacin treatment, depending on whether P. aeruginosa and S. aureus were growing in mono- or coculture biofilms, was used to validate our in vitro coculture conditions.
Note: Reproducció del document publicat a: https://doi.org/10.1038/s41598-019-52726-0
It is part of: Scientific Reports, 2019, vol. 9, p. 16284
URI: http://hdl.handle.net/2445/162617
Related resource: https://doi.org/10.1038/s41598-019-52726-0
ISSN: 2045-2322
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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