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Title: Multi-level immune response network in mild-moderate Chronic Obstructive Pulmonary Disease (COPD)
Author: Cruz Santa Cruz, Tamara
López Giraldo, Alejandra
Noell, Guillaume
Casas Recasens, Sandra
Garcia, Tamara
Molins López-Rodó, Laureano
Juan, Manel
Fernandez, M.A.
Agustí García-Navarro, Àlvar
Faner, Rosa
Keywords: Bronquitis
Malalts crònics
Hàbit de fumar
Cèl·lules immunocompetents
Chronically ill
Immunocompetent cells
Issue Date: 12-Jul-2019
Publisher: BioMed Central
Abstract: Background: Chronic Obstructive Pulmonary Disease (COPD) is associated with an abnormal pulmonary and systemic immune response to tobacco smoking. Yet, how do immune cells relate within and between these two biological compartments, how the pulmonary infiltrate influences the lung transcriptome, and what is the role of active smoking vs. presence of disease is unclear. Methods: To investigate these questions, we simultaneously collected lung tissue and blood from 65 individuals stratified by smoking habit and presence of the disease. The immune cell composition of both tissues was assessed by flow cytometry, whole lung transcriptome was determined with Affymetrix arrays, and we used Weighted Gene Co-expression Network Analysis (WGCNA) to integrate results. Results: Main results showed that: (1) current smoking and the presence of COPD were both independently associated with a reduction in the proportion of lung T cells and an increase of macrophages, specifically those expressing CD80 + CD163+; (2) changes in the proportion of infiltrating macrophages, smoking status or the level of airflow limitation were associated to different WGCNA modules, which were enriched in iron ion transport, extracellular matrix and cilium organization gene ontologies; and, (3) circulating white blood cells counts were correlated with lung macrophages and T cells. Conclusions: Mild-moderated COPD lung immune infiltrate is associated with the active smoking status and presence of disease; is associated with changes in whole lung tissue transcriptome and marginally reflected in blood.
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It is part of: Respiratory Research, 2019, vol. 20, p. 152
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ISSN: 1465-993X
Appears in Collections:Articles publicats en revistes (Medicina)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

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