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http://hdl.handle.net/2445/164827
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DC Field | Value | Language |
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dc.contributor.author | Marín, Sandra | - |
dc.contributor.author | Estil·les Altimiras, Elisabet | - |
dc.contributor.author | Lladó Garriga, Laura | - |
dc.contributor.author | San José, Patricia | - |
dc.contributor.author | Nacher, Montserrat | - |
dc.contributor.author | Téllez i Besolí, Noèlia | - |
dc.contributor.author | Montanya Mias, Eduard | - |
dc.date.accessioned | 2020-06-08T15:38:04Z | - |
dc.date.available | 2020-06-08T15:38:04Z | - |
dc.date.issued | 2019-07-19 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2445/164827 | - |
dc.description.abstract | Aim: To evaluate the effect of pancreatic ductal cells on experimental human islet transplantation. Materials and methods: Isolated islets were additionally purified by handpicking. Ductal cells were purified by magnetic cell sorting and then clustered into ductal pancreatospheres (DPS). Islets, DPS, and islets + DPS (100 islets + 75 DPS, or 100 islets + 200 DPS) were cultured and glucose-stimulated insulin secretion, β-cell apoptosis, and gene expression was determined. Islets and islets + DPS preparations (800 islets + 600 DPS) were transplanted to streptozotocin-treated immunodeficient mice and glycemia, graft morphometry, and gene expression were determined. Results: Insulin stimulation index was higher in islets than in islets co-cultured with DPS (5.59 ± 0.93 vs 4.02 ± 0.46; p<0.05). IL1B and CXCL11 expression was higher in 100 islets + 200 DPS than in islets (p<0.01), and IL-1β was detected in supernatants collected from DPS and islets + DPS preparations, but not in islets. Hyperglycemia developed in 33% and 67% of mice transplanted with islets or with islets + DPS respectively. β-cell mass was 26% lower in islets + DPS than in islets grafts (p>0.05), and the ratio β-/endocrine non-β-cell mass was lower in islets + DPS grafts (islets: 2.05 ± 0.18, islets + DPS: 1.35 ± 0.15; p<0.01). IL1B and IL1RN expression was significantly higher in islets + DPS grafts. Conclusions: Islet preparations enriched with ductal cells have a lower insulin stimulation index in vitro and achieved a worse metabolic outcome after transplantation. Inflammation may mediate the deleterious effects of ductal cells on islet cells. | - |
dc.format.extent | 16 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0220064 | - |
dc.relation.ispartof | PLoS One, 2019, vol. 14, num. 7, p. e0220064 | - |
dc.relation.uri | https://doi.org/10.1371/journal.pone.0220064 | - |
dc.rights | cc-by (c) Marín, Sandra et al., 2019 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Ciències Clíniques) | - |
dc.subject.classification | Illots de Langerhans | - |
dc.subject.classification | Insulina | - |
dc.subject.other | Islands of Langerhans | - |
dc.subject.other | Insulin | - |
dc.title | Pancreatic ductal cells may have a negative effect on human islet transplantation. PLoS One. 2019 Jul 19;14(7):e0220064. | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 695233 | - |
dc.date.updated | 2020-06-08T15:38:04Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) Articles publicats en revistes (Ciències Clíniques) |
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695233.pdf | 1.39 MB | Adobe PDF | View/Open |
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