Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/165168
Title: Biomechanical Response of Lung Epithelial Cells to Iron Oxide and Titanium Dioxide Nanoparticles
Author: Oliveira, Vinicius R.
Uriarte, Juan José
Falcones, Bryan
Jorba, Ignasi
Zin, Walter Araujo
Farré Ventura, Ramon
Navajas Navarro, Daniel
Almendros López, Isaac
Keywords: Epiteli
Pulmó
Contaminació atmosfèrica
Nanopartícules
Mecànica humana
Epithelium
Lung
Atmospheric pollution
Nanoparticles
Human mechanics
Issue Date: 16-Aug-2019
Publisher: Frontiers Media
Abstract: Increasing evidence shows that lungs can be damaged by inhalation of nanoparticles (NPs) at environmental and occupational settings. Recent findings have associated the exposure to iron oxide (Fe2O3) and titanium dioxide (TiO2) - NPs widely used in biomedical and clinical research - with pulmonary oxidative stress and inflammation. Although changes on cellular mechanics could contribute to pulmonary inflammation, there is no information regarding the effects of Fe2O3 and TiO2 on alveolar epithelial cell biomechanics. The aim was to investigate the NPs-induced biomechanical effects in terms of cell stiffness and traction forces exerted by human alveolar epithelial cells. Cell Young's modulus (E) measured by atomic force microscopy in alveolar epithelial cells significantly decreased after exposure to Fe2O3 and TiO2 (∼28 and ∼25%, respectively) compared to control conditions. Moreover, both NPs induced a similar reduction in the traction forces exerted by the alveolar epithelial cells in comparison to the control conditions. Accordingly, immunofluorescence images revealed a reduction of actomyosin stress fibers in response to the exposure to NPs. However, no inflammatory response was detected. In conclusion, an acute exposure of epithelial pulmonary cells to Fe2O3 and TiO2 NPs, which was mild since it was non-cytotoxic and did not induce inflammation, modified cell biomechanical properties which could be translated into damage of the epithelial barrier integrity, suggesting that mild environmental inhalation of Fe2O3 and TiO2 NPs could not be innocuous.
Note: Reproducció del document publicat a: https://doi.org/10.3389/fphys.2019.01047
It is part of: Frontiers in Physiology, 2019, vol. 10, p. 1047
URI: http://hdl.handle.net/2445/165168
Related resource: https://doi.org/10.3389/fphys.2019.01047
ISSN: 1664-042X
Appears in Collections:Articles publicats en revistes (Biomedicina)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

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