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Title: Assessment of Developmental Delay in the Zebrafish embryo Teratogenicity Assay
Author: Teixidó Condomines, Elisabet
Piqué Benages, Maria Esther
Gómez Catalán, Jesús
Llobet Mallafré, Joan M. (Joan Maria)
Keywords: Enzimologia
Peix zebra
Human abnormalities
Zebra danio
Issue Date: 27-Feb-2013
Publisher: Elsevier Ltd
Abstract: In this study we analyzed some aspects of the assessment of developmental delay in the zebrafish embryotoxicity/teratogenicity test and explored the suitability of acetylcholinesterase (AChE) activity as a biochemical marker and as a higher throughput alternative to morphological endpoints such as head-trunk angle, tail length and morphological score. Embryos were exposed from 4 to 52 h post-fertilization (hpf) to a selection of known embryotoxic/teratogen compounds (valproic acid, retinoic acid, caffeine, sodium salicylate, glucose, hydroxyurea, methoxyacetic acid, boric acid and paraoxon-methyl) over a concentration range. They were evaluated for AChE activity, head-trunk angle, tail length and several qualitative parameters integrated in a morphological score. In general, the different patterns of the concentration-response curves allowed distinguishing between chemicals that produced growth retardation (valproic and methoxyacetic acid) and chemicals that produced non-growth-delay related malformations. An acceptable correlation between the morphological score, AChE activity and head-trunk angle as markers of developmental delay was observed, being AChE activity particularly sensitive to detect delay in the absence of malformations.
Note: Versió postprint del document publicat a:
It is part of: Toxicology in Vitro, 2013, vol. 27, num. 1, p. 469-478
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ISSN: 0887-2333
Appears in Collections:Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)

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